小檗碱预处理对大鼠肝脏冷缺血再灌注时肠损伤的影响  

Effect of berberine preconditioning on intestinal injury caused by liver cold ischemia/reperfusion in rats

作　　者：刘文娜[1] 杜洪印[2] 喻文立[2] 翁亦齐[2] 王永旺[2] 王玉亮[3] 

机构地区：[1]天津医科大学一中心临床学院,300192 [2]天津市第一中心医院麻醉科 [3]卫生部危重病急救医学重点实验室

出　　处：《中华麻醉学杂志》2014年第11期1312-1315,共4页Chinese Journal of Anesthesiology

基　　金：天津市卫生行业重点攻关项目（12KG101）

摘　　要：目的 探讨小檗碱预处理对大鼠肝脏冷缺血再灌注时肠损伤的影响.方法 清洁级健康雄性SD大鼠24只,体重220～ 250 g,采用随机数字表法分为3组（n=8）：假手术组（S组）、肝脏冷缺血再灌注组（I/R组）和小檗碱预处理组（B组）.B组于术前7d开始经胃灌注小檗碱200 mg/kg,1次/d,连续7d.I/R组给予等量生理盐水.于术后8h时,留取肠组织,计算湿/干重比（W/D比）,进行肠组织损伤评分,测定肠组织MDA和SOD水平、细胞色素C、Bax和Bcl-2的表达和凋亡细胞计数.结果 与S组比较,I/R组和B组肠组织损伤评分、W/D比、MDA含量和凋亡细胞计数升高,SOD活性降低,细胞色素C和Bax表达上调,Bcl-2表达下调（P＜0.05）;与I/R组比较墙组肠组织损伤评分、W/D比、MDA含量和凋亡细胞计数降低,SOD活性升高,细胞色素C和Bax表达下调,Bcl-2表达上调（P＜0.05）.结论 小檗碱预处理可减轻大鼠肝脏冷缺血再灌注时的肠损伤,机制与抑制脂质过氧化反应和细胞凋亡有关.Objective To investigate the effect of berberine preconditioning on the intestinal injury caused by liver cold ischemia/reperfusion （I/R） in rats.Methods Twenty-four pathogen-free male Sprague-Dawley rats,weighing 220-250 g,were randomized into 3 groups （n =8 each） using a random number table：sham operation group （S group）,I/R group,and berberine preconditioning group （B group）.The animals were anesthetized with 5％ chloral hydrate 60 mg/kg.In B group,berberine 200 mg/kg was administered through a gastric tube once a day for 7 consecutive days starting from 7 days before operation.The equal volume of normal saline was given instead of berberine in group Ⅰ/R.At 8 h after operation,the rats were sacrificed and the intestines were harvested for determination of wet/dry lung weight ratio （W/D ratio）,levels of malondialdehyde （MDA） and superoxide dismutase （SOD）,expression of cytochrome C,Bax and Bcl-2 and apoptotic cell count,and for microscopic examination.Intestinal damage was assessed and scored according to Chiu.Results Compared with group S,Chiu＇s score,W/D ratio,MDA content and apoptotic cell count were significantly increased,SOD activity was decreased,the expression of cytochrome C and Bax was up-regulated,and the expression of Bcl-2 was downregulated in I/R and B groups.Compared with group I/R,Chiu＇s score,W/D ratio,MDA content and apoptotic cell count were significantly decreased,SOD activity was increased,the expression of cytochrome C and Bax was down-regulated,and the expression of Bcl-2 was up-regulated in B group.Conclusion Berberine preconditioning can reduce the intestinal injury caused by liver cold I/R in rats,and the mechanism is related to inhibition of lipid peroxidation and cell apoptosis.

关 键 词：小檗碱 再灌注损伤 肝 肠 

分 类 号：R657.3[医药卫生—外科学]