远隔缺血后处理对大鼠心肌缺血再灌注时脂联素水平的影响  

Effect of remote limb ischemic postconditioning on level of adiponectin during myocardial ischemia-reperfusion in rats

作　　者：谢海辉[1] 张曙[1] 李知平[1] 李恒昌[2] 许立新[2] 杜魏 叶志彬[3] 

机构地区：[1]南方医科大学附属东莞市人民医院麻醉科,523018 [2]广州医科大学附属广州市第一人民医院麻醉科 [3]南方医科大学附属东莞市人民医院心胸外科

出　　处：《中华麻醉学杂志》2014年第11期1386-1388,共3页Chinese Journal of Anesthesiology

基　　金：广东省医学科研基金项目（A2012786）

摘　　要：目的 探讨远隔缺血后处理对大鼠心肌缺血再灌注时脂联素水平的影响.方法 健康清洁级雄性SD大鼠57只,8周龄,体重250 ～ 350 g,采用随机数字表法分为3组（n=19）：假手术组（S组）、缺血再灌注组（I/R组）和远隔缺血后处理组（RLIP组）.采用结扎左冠状动脉30 min后恢复血流再灌注180 min的方法制备心肌缺血再灌注损伤模型.RLIP组于结扎左冠状动脉20 min时实施远隔缺血后处理：结扎双后肢大腿中、上1/3部位10 min恢复血流再灌注.再灌注30、60、120及180 min时记录ST段幅度.于再灌注180 min时,采集颈静脉血样3ml,检测血清CK-MB活性及cTnI浓度;取心肌组织测定心肌梗死体积（IS）,采用ELISA法检测心肌组织脂联素含量.结果 与S组比较,I/R组和RLIP组再灌注各时点ST段抬高,血清CK-MB活性及cTnI浓度和IS升高,心肌组织脂联素含量降低（P＜0.05）;与I/R组比较,RLIP组再灌注各时点ST段抬高程度降低,血清CK-MB活性及cTnⅠ浓度和IS降低,心肌组织脂联素含量升高（P＜0.05）.结论 远端缺血后处理减轻大鼠心肌缺血再灌注损伤的机制与增加心肌脂联素水平有关.Objective To evaluate the effect of remote limb ischemic postconditioning on the level of adiponectin during myocardial ischemia-reperfusion （I/R） in rats.Methods Fifty-seven male Sprague-Dawley rats,aged 8 weeks,weighing 250-350 g,were randomly divided into 3 groups （n =19 each）：sham operation group （group S）; group I/R; remote limb ischemic postconditioning group （group RLIP）.Myocardial ischemia was induced by occlusion of anterior descending branch of left coronary artery for 30 min followed by 180 min reperfusion in urethane-anesthetized rats.In group S,the anterior descending branch was only exposed but not ligated.The animals underwent 10 min ischemia of bilateral hind limbs starting from 20 min of occlusion of the anterior descending branch,followed by reperfusion in RLIP group.The changes in S-T segment were recorded at 30,60,120 and 180 min of reperfusion.At 180 min of reperfusion,the blood samples were taken from the jugular vein for determination of serum levels of creatine kinase MB （CK-MB） and cardiac troponin Ⅰ （cTnⅠ）.The animals were then sacrificed and hearts were removed for measurement of myocardial infarct size （IS） and adiponectin contents in myocardial tissues （by ELISA）.Results Compared with group S,S-T segment was significantly elevated at each time point,myocardial IS,and serum cTnⅠ and CK-MB levels were increased,and adiponectin contents were decreased in I/R and RLIP groups.Compared with group I/R,elevation of S-T segment was significantly decreased at each time point,myocardial IS,and serum cTnⅠ and CK-MB levels were decreased,and adiponectin contents were increased in RLIP group.Conclusion The mechanism by which remote limb ischemic postconditioning reduces myocardial I/R injury is related to increased level of adiponectin in the myocardium of rats.

关 键 词：脂联素 心肌再灌注损伤 四肢 缺血后处理 

分 类 号：R542.2[医药卫生—心血管疾病]