Tightening up the structure, lighting up the pathway:application of molecular constraints and light to manipulate protein folding, self-assembly and function  

作　　者：MARKIEWICZ Beatrice N. CULIK Robert M. GAI Feng 

机构地区：[1]Department of Chemistry, University of Pennsylvania [2]Department of Biochemistry and Biophysics, University of Pennsylvania

出　　处：《Science China Chemistry》2014年第12期1615-1624,共10页中国科学（化学英文版）

基　　金：supported by the National Institutes of Health(GM-065978,AG-039253)

摘　　要：Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and proteins.Recently,other types of molecular constraints,especially photoresponsive linkers and functional groups,have also found increased use in a wide variety of applications.Herein,we provide a concise review of using various forms of molecular strategies to constrain proteins,thereby stabilizing their native states,gaining insight into their folding mechanisms,and/or providing a handle to trigger a conformational process of interest with light.The applications discussed here cover a wide range of topics,ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function.Chemical cross-linking provides an effective avenue to reduce the conformational entropy of polypeptide chains and hence has become a popular method to induce or force structural formation in peptides and proteins.Recently,other types of molecular constraints,especially photoresponsive linkers and functional groups,have also found increased use in a wide variety of applications.Herein,we provide a concise review of using various forms of molecular strategies to constrain proteins,thereby stabilizing their native states,gaining insight into their folding mechanisms,and/or providing a handle to trigger a conformational process of interest with light.The applications discussed here cover a wide range of topics,ranging from delineating the details of the protein folding energy landscape to controlling protein assembly and function.

关 键 词：protein folding aggregation SELF-ASSEMBLY CROSS-LINKER phototrigger light-activation 

分 类 号：O629.73[理学—有机化学]