Bruker Biotyper和Vitek MS系统质谱分析鉴定革兰阴性菌临床分离株的比较研究  

Comparison of Bruker Biotyper system and Vitek MS system in the identification of clinical Gram-negative isolates

作　　者：王璐[1] 杨柳[1] 任微[1] 褚美玲[1] 孟冬娅[1] 薛文成[1] 

机构地区：[1]沈阳军区总医院检验科,110016

出　　处：《传染病信息》2014年第5期282-285,295,共5页Infectious Disease Information

摘　　要：目的比较并评价两种基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF-MS)系统——Bruker MALDI Biotyper系统(以下简称Bruker Biotyper系统)和MALDITOF Vitek MS系统(以下简称Vitek MS系统)在革兰阴性菌临床分离株鉴定中的应用。方法收集沈阳军区总医院2012年3月—2013年1月分离自血液、尿液、脑脊液、分泌物、伤口拭子和痰液临床标本的革兰阴性菌共120株。应用Vitek 2 Compact生化鉴定系统将每株细菌鉴定到种,而后采用Bruker Biotyper和Vitek MS系统对其进行鉴定,三者鉴定结果存在差异的菌株经16S rDNA测序最终确认菌种。结果对于120株革兰阴性菌临床分离株,Bruker Biotyper和Vitek MS系统在属的水平上正确鉴定率分别为95.0%和92.5%,在种的水平上正确鉴定率分别为89.2%和86.7%,差异均无统计学意义。结论 Bruker Biotyper和Vitek MS系统对革兰阴性菌临床分离株的正确鉴定率不存在差异,两种系统的鉴定结果与各自的图谱数据库有密切关系。Objective To compare and evaluate the use of two matrix-assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS） systems, Bruker MALDI Biotyper system（Bruker Biotyper system） and MALDI-TOF Vitek MS system（Vitek MS system） in the identification of Gram-negative clinical isolates. Methods A total of 120 Gram-negative isolates were collected from blood, urine, cerebrospinal fluid, secretion, wound swab and sputum samples from patients treated in General Hospital of Shenyang Military Area Command from Mar. 2012 to Jan. 2013. Each isolate was firstly identified to the species by Vitek 2Compact system, and then identified by Bruker Biotyper system and Vitek MS system. Isolates differently identified by the three systems were finally confirmed by 16 S rDNA sequencing as the gold standard. Results Of the 120 isolates, 95.0% were correctly identified to the genus level by Bruker Biotyper system and 92.5% by Vitek MS system, and 89.2% were correctly identified to the species level by Bruker Biotyper system and 86.7% by Vitek MS system, and the differences between them were not significant. Conclusions Bruker Bio-typer system and Vitek MS system were not significantly different in the accuracy rates of identifying clinical Gram-negative isolates.In addition, the identification results of the two systems are closely related to their own databases of pictorial works.

关 键 词：光谱法 质量 基质辅助激光解吸电离基质辅助激光解吸电离飞行时间质谱 图谱 革兰阴性菌 

分 类 号：R313[医药卫生—基础医学]