脑缺血再灌注大鼠脑内Mrp1的表达变化  

The Changes of Multidrug Resistance Protein-1 Expression in Brain after Ischemia-reperfusion in Rats

作　　者：杨力[1] 李洁[1] 李志雄[2] 容伟[3] 于建云[2] 郭泽云[1] 

机构地区：[1]昆明医科大学人体解剖学与组织胚胎学系,云南昆明650500 [2]昆明医科大学法医学院,云南昆明650500 [3]昆明医科大学第二附属医院神经内科,云南昆明650101

出　　处：《昆明医科大学学报》2014年第10期4-7,共4页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81060101);云南省科技厅基金资助项目(2013FZ056);云南省教育厅基金资助项目(2011Y168)

摘　　要：目的观察脑缺血再灌注后多药耐药蛋白(Mrp1)在脑内的表达变化,探讨其与缺血再灌注的关系.方法 24只大鼠随机分为假手术组(n=6)和缺血再灌注后1、3、7d组(n=6).应用颈内动脉线栓法建立大鼠大脑中动脉缺血再灌注模型,以抗Mrp1抗体进行免疫组化染色,检测缺血再灌注后脑组织中Mrp1的表达变化.结果 Mrp1在正常脑内的神经元及胶质细胞中均有表达.缺血再灌注后1d、3 d、7d组中,缺血再灌注区的皮质、海马及纹状体内可见Mrp1表达升高,阳性细胞数量增多,在3d组达高峰(P<0.05).结论大鼠脑缺血再灌注后细胞中Mrp1的表达增高,可能是细胞受损时的自我保护反应,但同时也可能与临床耐药性的发生有关.Objective To explore the expression changes of multidrug resistance protein-1 in brain ischemia/reperfusion in rats.Methods The models of ischemia-reperfusion were established by focal middle cerebral artery occlusion（MCAO） method. 24 adult male SD rats were randomly divided into sham and ischemia/reperfusion group（I/F） including 1day,3days,7days after I/F（n=6）;the expression of Mrp1 was observed by immunohistochemistry.Results Mrp1 was expressed in cells in cerebral cortex, hippocampus and corpus striatum of normal brain and ischemia/ reperfusion brain. The over expression of Mrp1 was found in infarct region after I/R,especially in 3 d group（P〈0.05）.Conclusions The over expression of mrp1 can be found in brain after I/R. It suggests that over expression of mrp1 induced by ischemia/ reperfusion injuring brain tissue may be related with neural self-protection or multidrug resistance.

关 键 词：脑缺血再灌注 MRP1 大鼠 

分 类 号：R277.733.1[医药卫生—中医学]