拉应力对体外培养人足跟皮肤成纤维细胞增殖影响的实验研究  

Effect of tensile stress on human heel skin fibroblast proliferation in vitro

作　　者：李俊华[1] 黄忠名[1] 杜少华[1] 黄利刚[1] 

机构地区：[1]杭州市萧山区中医院,浙江杭州311200

出　　处：《中国骨伤》2014年第10期838-842,共5页China Journal of Orthopaedics and Traumatology

基　　金：浙江省医药卫生科技计划(编号:2012KYB169;2013KYB226);浙江省中医药优秀青年人才基金计划(编号:2012ZQ023);浙江省公益性技术应用研究计划(编号:2013c33096);杭州市医学重点专科专病项目计划(编号:20120533Q39);杭州市卫生科技计划(编号:2013B51);杭州市萧山区重点科研项目(编号:2012234)~~

摘　　要：目的:观察拉应力对人足跟外侧皮肤成纤维细胞增殖的影响,为预防跟骨骨折术后切口边缘皮肤坏死、切口不愈合提供一定的理论依据。方法:取40岁男性足跟外侧皮肤成纤维细胞培养及传代后,分0 h组、6 h组、24 h组进行拉应力加载试验,检测培养液中TGF-β1、IL-6含量,使用透射电镜、光镜观察线粒体、细胞核的变化。结果:在10%的拉应力下,成纤维细胞随着承受应力的时间增加,线粒体逐渐减少,TGF-β1和IL-6的分泌受到抑制,细胞增殖逐渐受到抑制。结论:人足跟外侧皮肤长时间处于拉应力状态是跟骨骨折手术后切口边缘皮肤坏死、切口不愈合的重要原因。Objective：To observe the effect of tensile stress on human heel skin fibroblast proliferation in vitro,providing a theoretical basis for preventing the wound edge skin necrosis and nonunion after calcaneal fracture surgery.Methods：Fibroblast cells were taken from lateral heel skin of a 40 year-old-man,then cultured and subcultured in vitro.After that,they were divided into three groups：0 hours group,6 hours group and 24 hours group and were tested by tensile stress testing.The levels of TGF-β1 and IL-6 in nutrient fluid were measured.Transmission electron microscope and light microscope was applied for observe mitochondria and nucleus.Results：Under 10％ of the tensile stress,mitochondria decreased,the levels of TGF-β1 and IL-6 in nutrient fluid were decreased and cell proliferation was inhibited gradually with time increasing.Conclusion：The human lateral heel skin in a long-time tensile stress state is an important cause of wound edge skin necrosis and nonunion after calcaneus fracture surgery.

关 键 词：成纤维细胞 跟骨 骨折 皮肤 手术后并发症 拉应力 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]