生、熟大黄泻下作用及其机制研究  

Purgative Activity Difference and Mechanism of Raw and Processed Rhei Radix et Rhizoma

作　　者：吴晓青[1] 胡昌江[1] 赵玲[1] 耿媛媛[1] 潘新[1] 胡麟[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《中药材》2014年第9期1562-1565,共4页Journal of Chinese Medicinal Materials

基　　金：中医药行业科研专项(20110700710)

摘　　要：目的:研究生、熟大黄泻下作用,并探讨其作用机制。方法:取雄性小鼠,造成热结便秘模型后,分为模型组、通便灵胶囊组(1 g/kg)、生大黄组(5.00、2.50、1.25 g/kg)、熟大黄组(5.00、2.50、1.25 g/kg),观察生、熟大黄泻下作用差异。又取造模成功后大鼠分为模型组、通便灵胶囊组(0.5 g/kg)、生大黄组(1.25 g/kg)、熟大黄组(1.25 g/kg),取血清和结肠检测胃肠激素和肠神经递质水平的变化。结果:生大黄与熟大黄比较EI(泻下指数)显著增高(P<0.05)。生大黄组与熟大黄组相比,血清GAS、MTL、VIP、NT、SS、Ach E、SP、ET-1和结肠GAS、MTL、VIP的含量差异均有统计学意义(P<0.01)。结论:生大黄和熟大黄对胃肠激素和肠神经递质调控作用具有明显差异,这可能是大黄"生泻熟缓"的作用机制之一。Objective： To investigate the purgative activity difference and mechanism of raw and processed Rhei Radix et Rhizoma. Methods： Mail mice were divided into 9 groups： normal group（ saline）,model group,Tongbianling group（ 1 g / kg）,raw Rhei Radix et Rhizoma groups（ 5. 00,2. 50 and 1. 25 g / kg）,processed Rhei Radix et Rhizoma groups（ 5. 00,2. 50 and 1. 25 g / kg）. After oral administration for 5 days,the diarrhea experiment and intestinal propelling test were carried out to evaluate the purgative effect difference of raw and processed Rhei Radix et Rhizoma. Wistar rats were divided into normal group（ normal saline）,model group,Tongbianling group（ 0. 5 g / kg）,raw Rhei Radix et Rhizoma group（ 1. 25 g / kg）,processed Rhei Radix et Rhizoma group（ 1. 25 g / kg）. The changes of levels of gastin（ GAS）,motilin（ MTL）,vasoactive peptide（ VIP）,neurotensin（ NT）,somatostatin（ SS）,acetylcholinesterase（ Ach E）,substance P（ SP） and endothelin（ ET-1） in blood of the rats and gastin（ GAS）,motilin（ MTL）,vasoactive peptide（ VIP） and neurotensin（ NT） in colon of the rats were detected. Result： The levels of GAS,MTL,VIP,NT,SS,Ach E,SP and ET-1 in serum of raw Rhei Radix et Rhizoma group,and GAS,MTL and VIP in colon of raw Rhei Radix et Rhizoma group were different obviously comparing with the processed one（ P〈0. 01）. Conclusion： The raw Rhei Radix et Rhizoma and the processed one had obvious differences in regulating intestinal gastrointestinal hormone and neurotransmitter. This effect may be the reason or one of the reasons for purgative activity difference between raw and processed Rhei Radix et Rhizoma.

关 键 词：生大黄 熟大黄 泻下作用 胃肠激素 肠神经递质 

分 类 号：R283.3[医药卫生—中药学] R285.5[医药卫生—中医学]