指纹图谱与一测多评法相结合测定五味子种子中木脂素类成分  

Determination of Lignanoids in Seeds of Schisandra chinensis by Combinative Methods of Fingerprint and QAMS

作　　者：窦志华[1] 安莉萍[2] 罗琳[3] 居宇峰[1] 乔进[1] 陈敏[1] 缪应祥[1] 

机构地区：[1]南通大学附属南通第三医院,江苏南通226006 [2]南京中医药大学,江苏南京210023 [3]南通大学,江苏南通226019

出　　处：《中药材》2014年第9期1604-1608,共5页Journal of Chinese Medicinal Materials

基　　金：南通市应用研究计划项目(K2008027);江苏省药学会科研项目(JS2012-04)

摘　　要：目的:建立指纹图谱及一测多评法测定五味子种子中6个木脂素类成分含量的方法。方法:采用高效液相色谱法测定11批五味子种子的指纹图谱,建立指纹图谱共有模式。以五味子醇甲为内参物,建立五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素与五味子醇甲的相对校正因子,并进行含量计算,实现一测多评。同时采用外标法测定11批五味子种子中6个木脂素类成分的含量,比较计算值与实测值的差异,验证一测多评法的准确性。结果:11批五味子种子指纹图谱标定了共有峰24个,指认了其中6个木脂素类成分。11批样品指纹图谱与对照指纹图谱的相似度均大于0.980。建立的相对校正因子重现性良好,采用校正因子计算的含量值与实测值之间无显著差异。结论:所建立的方法准确、可行,可用于五味子种子中木脂素类成分的定性定量分析。Objective： To establish a fingerprint of seeds of Schisandra chinensis（ SSC） and develop a method of quantitative analysis of multi-components by single marker（ QAMS） for simultaneous determining six lignanoids in SSC. Methods： Eleven batches of SSC were determined by HPLC and a common mode of fingerprint has been established. A method was developed for QAMS to determine schizandrol A,schizandrol B,schisantherin A,deoxyschizandrin,schizandrin B and schizandrin C in SSC. Schizandrol A was selected as internal reference; the relative correction factors（ RCF） of other five lignanoids to the internal reference were calculated. The contents of the six lignanoids in eleven batches of SSC were determined by both external standard method and QAMS. The QAMS method was evaluated by comparison of its assay results with that of external standard method. Results： There were 24 common peaks in fingerprints of eleven batches of SSC,six of them were identified. The similarities of fingerprints of eleven batches of SSC were over 0. 980. The established RCF had a good reproducibility. No significant differences were found between the quantitative results of external standard method and QAMS. Conclusion： The developed method is accurate,feasible,and can be used for the qualitative and quantitative analysis of lignanoids in SSC.

关 键 词：五味子种子 指纹图谱 一测多评法 木脂素 

分 类 号：R284.1[医药卫生—中药学]