富硒栽培丹参中硒的赋存形态研究  

Study on Binding Forms of Selenium in Selenium-enriched Salvia miltiorrhiza

作　　者：胡晓荣[1] 田丽[1] 董维兵[1,2] 胡浩[1] 

机构地区：[1]矿产资源化学四川省高等学校重点实验室/成都理工大学材料与化学化工学院,四川成都610059 [2]成都市新都区疾病预防控制中心,四川成都610500

出　　处：《中药材》2014年第9期1609-1612,共4页Journal of Chinese Medicinal Materials

基　　金：四川省科技厅应用基础项目(07JY029-114);国家大学生创新创业训练计划项目(14Z003-38)

摘　　要：目的:研究富硒丹参中硒与蛋白质、多糖和核酸结合的含量分布以及丹参对无机硒化合物的生物转化效率。方法:采用不同溶剂分别提取富硒丹参中的蛋白质、多糖和核酸;应用光度法测定蛋白质、多糖和核酸含量,氢化物-原子荧光法测定硒含量。结果:蛋白质结合硒占总硒的74.2%,多糖结合硒占总硒的39.5%,核酸结合硒占总硒的2.3%。在水、Na Cl溶液、乙醇溶液和Na OH溶液连续浸提的组分中,水溶性蛋白硒的含量最高,占总硒的46.0%。弱酸性和弱碱性磷酸缓冲溶液提取的蛋白质中,弱碱溶性蛋白中硒含量更高,占总硒的51.2%。结论:富硒丹参中硒主要以含硒蛋白和含硒多糖的形式存在。丹参富硒栽培能有效实现无机硒的有机化。Objective： To understand the absolute and relative concentrations of proteins,polysaccharides and nucleic acids that are Selenium-binding in Selenium-enriched Salvia miltiorrhiza,and to determine the efficiency of biotransformation of inorganic Selenium compounds in Salvia miltiorrhiza. Methods： Extract the Selenium-binding proteins,polysaccharides and nucleic acids in Selenium-enriched Salvia miltiorrhiza using different solvents. Determine the concentrations of proteins,polysaccharides and nucleic acids by spectrophotometry,the concentration of Selenium by Hydride generation atomic fluorescence spectrometry. Results： Selenium-binding proteins took up 74. 2% of the total amount of Selenium in the tested Salvia miltiorrhiza,while Selenium-binding polysaccharides took up 39. 5%and Selenium-binding nucleic acids took up 2. 3%. Selenium that was bound with the water-soluble proteins came out as the most concentrated,taking up 46. 0% of the total amount of Selenium,during the extraction of Selenium using water,Na Cl,ethanol and Na OH solution,respectively. Being extracted by the weak acid and alkali phosphoric acid buffer solutions,Selenium-binding proteins were more concentrated in the alkali buffer solution,taking up 51. 2% of the total amount. Conclusions： In Salvia miltiorrhiza,Selenium exists mainly in the forms of selenium-binding proteins and Selenium-binding polysaccharides. Cultivation of Selenium-enriched Salvia miltiorrhiza achieves the biotransformation of inorganic Selenium compounds into organic compounds efficiently.

关 键 词：富硒丹参 含硒蛋白 含硒多糖 含硒核酸 

分 类 号：R284.1[医药卫生—中药学]