HPLC法同时测定大鼠口服墨旱莲提取物后血浆中四种黄酮类化合物  

HPLC Determination of Four Flavonoids in Rat Plasma After Oral Ecliptae Herba Extract

作　　者：邓斌[1] 韦炳华[1] 黄盛超[1] 张志豪[1] 唐蕾[1] 

机构地区：[1]中山大学附属第一医院药学部,广东广州510080

出　　处：《中药材》2014年第9期1636-1640,共5页Journal of Chinese Medicinal Materials

基　　金：广州市重大科技专项计划项目(2008A1-E4101-7);广东省中医药局课题(2009159)

摘　　要：目的：建立同时测定大鼠口服旱莲草提取物后血浆中4种黄酮含量的HPLC法。方法：采用高效液相色谱法,色谱柱为Neocleodur 100-5 C18柱（250 mm×4.6 mm,5μm）,流动相A为甲醇-0.2%磷酸溶液（50∶50）,B为乙腈,梯度洗脱。流速为1.0 m L/min,检测波长为350 nm,柱温40℃。结果：木犀草素、芹菜素、野黄芩素和香叶木素在浓度为0.02-50μg/m L时与色谱峰面积比之间的线性关系良好,平均回收率为71.5%-90.2%。结论：该方法快速、简便、准确,可用于大鼠血浆中木犀草素、芹菜素、野黄芩素和香叶木素的HPLC测定及药动学研究。Objective： To establish an HPLC method for determination of four flavonoids in rat plasma after oral Ecliptae Herba extract. Methods： HPLC separation was carried out in a Neocleodur 100-5 C18column（ 250 mm × 4. 6 mm,5 μm） with mobile phase A（ consisting of methanol-0. 2% phosphoric acid = 50∶ 50） and mobile phase B（ acetonitrile） with gradient elution at the flow rate of 1. 0m L / min. The detection wavelength was 350 nm and the column temperature was maintained at 40 ℃. Results： There were good linear relationships between concentration and peak area ratio of luteolin,apigenin,scutellarein and diosmetin in the ranges of 0. 02 - 50 μg /m L with the recovery rates of 71. 5% - 90. 2%. Conclusion： This method is rapid,accurate and reproducible for determination of luteolin,apigenin,scutellarein and diosmetin in rat plasma and study on pharmacokinetics of Ecliptae Herba extract.

关 键 词：旱莲草提取物 木犀草素 芹菜素 野黄芩素 香叶木素 高效液相色谱法 血药浓度 

分 类 号：R285.5[医药卫生—中药学]