蛋白激酶C对致敏人气道平滑肌张力的影响  

Effect of protein kinase C on the tension of passively sensitized human airway smooth muscle

作　　者：程东军[1,2] 徐永健[3] 刘先胜[3] 赵丽敏[1,2] 熊盛道[3] 

机构地区：[1]郑州大学人民医院 [2]河南省人民医院呼吸内科,郑州450003 [3]华中科技大学同济医学院附属同济医院呼吸内科,武汉430030

出　　处：《中华实用诊断与治疗杂志》2014年第11期1057-1059,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(30270583)

摘　　要：目的探讨蛋白激酶C(protein kinase C,PKC)对体外被动致敏人气道平滑肌(human airway smooth muscle,HASM)张力的影响。方法行肺叶切除术患者6例,其中肺癌5例,支气管扩张1例,取手术切除的正常支气管组织标本分为哮喘组与对照组,哮喘组支气管组织用哮喘患者的血清致敏,对照组支气管组织用体检健康者的血清致敏。2组采用肌张力试验观察不同浓度组胺和PKC激活剂豆蔻酰佛波醇乙酯(Phorbol 12-myristate 13-acetate,PMA)对HASM张力的影响。2组均给予PMA阻断剂Ro31-8220和钙通道阻断剂硝苯地平,观察其对PMA所致HSAM收缩反应的抑制情况。结果哮喘组1×10-7、1×10-6、1×10-5、1×10-4 mol/L组胺作用的HASM张力((11.6±3.1)、(21.9±4.1)、(40.1±6.2)、(54.1±13.2)mg/mg)均较对照组((6.4±1.3)、(11.3±2.5)、(23.3±4.7)、(34.1±5.7)mg/mg)明显增高(P<0.05),1×10-7、5×10-7、1×10-6、5×10-6 mol/L PMA作用的HASM张力((12.1±2.7)、(30.9±7.0)、(41.2±9.2)、(53.1±12.3)mg/mg)均较对照组((8.6±1.9)、(22.8±4.8)、(30.9±5.4)、(38.3±7.9)mg/mg)明显增高(P<0.05);2组组胺和PMA作用的HASM张力均随浓度增高而增高,呈剂量依赖性;2组PMA所致HSAM的收缩反应均可被Ro31-8220和硝苯地平完全抑制。结论激活PKC可导致HASM张力增高,该效应在体外被动致敏的HASM中表现更明显。Objective To investigate the effect of protein kinase C（PKC）on the tension of passively sensitized human airway smooth muscle（HASM）.Methods Six patients undergoing pulmonary lobectomy included 5patients with pulmonary carcinoma and 1with bronchiectasis.The resected normal bronchial specimens were divided into asthma group and control group.Asthma group was sensitized by serum from asthmatic patients and control group was sensitized by serum from healthy volunteers.The effects of different concentrations of histamine and PKC activator Phorbol 12-myristate 13-acetate（PMA）on the tension of HASM were observed.Both two groups were administrated PMA inhibitor Ro31-8220 and calcium channel inhibitor nifedipine,and the depressions of Ro31-8220 and nifedipine on the constrictions of HASM were observed.Results The tensions of HASM in 1×10-7,1×10-6,1×10-5 and 1×10-4 mol/L of histamine were（11.6±3.1）,（21.9±4.1）,（40.1±6.2）,and（54.1±13.2）mg/mg in asthma group,significantly higher than those in control group（（6.4±1.3）,（11.3±2.5）,（23.3±4.7）,34.1±5.7）mg/mg）（P〈0.05）.The tensions of HASM in 1×10-7,5×10-7,1×10-6 and 5×10-6 of PMA were（12.1±2.7）,（30.9±7.0）,（41.2±9.2）and（53.1±12.3）mg/mg in asthma group,significantly higher than those in control group（（8.6±1.9）,（22.8±4.8）,（30.9±5.4）,（38.3±7.9）mg/mg）（P〈0.05）.Both histamine and tensions of HASM increased along with the concentrations in a dose-dependent tendency in two groups.The HASM constrictions from PMA administration of both groups were completely abolished by Ro31-8220 and nifedipine.Conclusion Activation of PKC could increase the tension of HASM,which is more notable in passively sensitized HASM rings.

关 键 词：哮喘 蛋白激酶C 人气道平滑肌张力 致敏 

分 类 号：R562.25[医药卫生—呼吸系统]