糖尿病小鼠视网膜中JNK3 mRNA表达的动态变化  

Dynamic expression of JNK3 mRNA in retina of diabetic mice by real-time quantitative PCR

作　　者：张曙光[1] 项杰 李燕[3] 袁援生[3] 马林昆[4] 

机构地区：[1]郑州市第二人民医院眼科,450000 [2]河南省直第三人民医院眼科,郑州450006 [3]昆明医科大学第一附属医院眼科,650031 [4]昆明医科大学第二附属医院眼科,650031

出　　处：《中华实验眼科杂志》2014年第11期994-997,共4页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景 糖尿病视网膜病变（DR）是糖尿病常见的眼部并发症,其发病机制与多种因素有关,c-jun N末端激酶（JNK）作为一种凋亡基因,在糖尿病的病理过程中发挥着重要作用,其研究已成为近些年的热点之一,而JNK3作为JNK的亚基因之一,在DR中的研究目前较少.目的 定量检测JNK3在糖尿病小鼠视网膜中的表达,探讨JNK3在DR中的作用.方法 选取SPF级6～8周龄C57BL/6雄性小鼠48只,适应性喂养1周后按照随机数字表法分为糖尿病组和正常对照组.30只糖尿病组小鼠用一次性腹腔内注射柠檬酸钠缓冲液溶解的质量分数1％链脲佐菌素（STZ）的方法诱导糖尿病模型,18只对照组小鼠腹腔内注射等容量柠檬酸钠缓冲液.分别于造模后2、4、8周摘除小鼠左眼眼球,提取视网膜组织,采用实时定量PCR法检测JNK3 mRNA在小鼠视网膜中表达的动态变化.结果 造模后2、4、8周,糖尿病组小鼠血糖水平明显高于正常对照组,差异均有统计学意义（t=-5.675、-5.498、-5.347,P＜0.01）.糖尿病组和正常对照组在造模后不同时间点小鼠视网膜中JNK3 mRNA相对表达量（A值）的差异均有统计学意义（F分组=102.345,P＜0.05;F时间 =131.679,P＜0.05）;其中造模后4周和8周糖尿病组小鼠视网膜中JNK3 mRNA相对表达量分别为3.21±0.14和5.43±0.37,均明显高于正常对照组的2.54 ±0.42和2.26±0.67,差异均有统计学意义（t=4.073、23.399,P＜0.05）;糖尿病组内比较可见,造模后8周小鼠视网膜中JNK3 mRNA相对表达量明显高于2周和4周值,差异均有统计学意义（t=10.756、16.857,P＜0.05）.结论 JNK3在糖尿病小鼠视网膜中表达量上调,其早期表达量随时间延长而增加.JNK3可能参与早期DR的发生和发展.Background Diabetic retinopathy （DR） is a common ocular complication of diabetes,and its pathogenesis is associated with a variety of factors.c-Jun N terminal kinase （JNK）,one of the genes involving in apoptosis,plays an important role in the pathology of diabetes,and relative research is catching increasing interests in recent years.Objective This study was to quantify the expression of JNK3 in retinas of DR murine.Methods Forty-eight SPF male C57BL/6 mice were randomly divided into the diabetes group and the normal control group.Diabetic mouse models were establishend by intraperitoneal injection of 1% streptozocin （STZ） dissolved by sodium citrate buffer,and equvilant volume of sodium citrate buffer was used in the same way in the mice of the control mice.The left eyeballs were obtained 2,4,8 weeks after modeling and the retinas were collected.Real-time quantitaive PCR was perfored to detect the expression of JNK3 mRNA in retinas.The use and care of the experimental mice complied with the Administration of Experimental Animals in Kunming Medical College.Results Blood glucose levels were significantly higher in 2,4,8 weeks after modeling in the diabetic group compared with the normal control group （t=-5.675,-5.498,-5.347,all at P〈0.01）.The relative expression levels of JNK3 mRNA （A value） in the retinas were significantly different between the groups at various time points （Fgroup =102.345,P〈0.05 ; Ftime =131.679,P〈 0.05）.The relative expression levels of JNK3 mRNA in the retinas were 3.21 ±0.14 and 5.43 ±O.37 in 4 and 8 weeks after modeling in the diabetic group,which were significantly elevated in comparison with the normal control group （2.54±0.42 versus 2.26±0.67） （t =4.073,23.399,both at P〈0.05）.Compared with the second week and fourth week,the relative expression levels of JNK3 mRNA in the retinas in the eighth week were significantly raised in the diabetic group （t =10.756,16.857,both at P 〈 0.05）.Conclusions JNK3 expression in the retina upregulates in d

关 键 词：糖尿病/并发症 视网膜 C57BL/6小鼠 c—Jun N末端激酶 实时定量PCR 

分 类 号：R587.2[医药卫生—内分泌]