Graves病患者外周血免疫调节细胞及其亚群比例异常  

Abnormal proportions of immune regulatory cells and their subsets in peripheral blood of patients with Graves' disease

作　　者：何珂[1] 胡蕴[1] 毛晓明[1] 

机构地区：[1]南京医科大学附属南京医院,南京市第一医院内分泌科,江苏南京210012

出　　处：《细胞与分子免疫学杂志》2014年第11期1190-1193,1197,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：南京市医学科技发展项目(ZKX12023)

摘　　要：目的观察Graves病(GD)患者外周血各种免疫调节细胞及其亚群的变化。方法采用流式细胞术(FCM)分别检测32例GD患者及30例健康人外周血调节性T细胞(Treg)、Th1细胞、Th2细胞、调节性B细胞(Breg)、树突状细胞(DC)等免疫细胞亚群,并进行比较。结果与正常对照组相比,GD患者Treg比例降低,Th1细胞、Th2细胞数量增加;Breg数量减少;DC数量下降,髓样DC(mDC)占DC比例下降,而浆细胞样DC(pDC)比例升高,成熟型DC比例升高。结论 GD患者外周血中存在免疫调节细胞的失衡,可能在GD的发生、发展中起一定作用。Objective To evaluate the changes of various immune regulatory cells and their subsets in peripheral blood mononuclear cells（ PBMCs） of patients with Graves＇ disease（ GD）. Methods Peripheral blood cells were obtained from32 GD patients and 30 normal subjects. The proportions of regulatory T cells（ Tregs）,T helper 1（ Th1） cells,Th2 cells,regulatory B cells（ Bregs）,dendritic cells（ DCs） and their subsets in PBMCs were analyzed with flow cytometry. Results Compared with the normal subjects,the proportions of Tregs,Bregs and total DCs significantly decreased in PBMCs of the GD patients. However,the proportions of Th1,Th2 cells in PBMCs significantly increased in the GD patients compared with the normal subjects. As for DC subsets,the proportion of myeloid DCs（ mDCs） dropped and plasmacytoid DCs（ pDCs）went up in PBMCs of the GD patients compared with the normal subjects. Moreover,the proportion of mature DCs was raised in total DCs in the GD patients compared with normal subjects. Conclusion There are disorders in various immune regulatory cells in PBMCs of the GD patients,which may play important roles in the onset and progression of GD.

关 键 词：GRAVES病 调节性T细胞 辅助性T细胞 调节性B细胞 树突状细胞 

分 类 号：R581.1[医药卫生—内分泌] R392.6[医药卫生—内科学]