干旱胁迫对转拟南芥Atpsy基因生菜的影响  

Effects of drought stress on Atpsy transgenic lettuce

作　　者：尤丽佳[1] 郭新波[2] 付雪晴[3] 唐岳立[3] 程海祺[3] 唐克轩[3] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]复旦大学生命科学学院,上海200433 [3]上海交通大学农业与生物学院,上海200240

出　　处：《上海农业学报》2014年第5期38-43,共6页Acta Agriculturae Shanghai

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB108805);上海市重点学科项目(B209)

摘　　要：干旱胁迫处理转拟南芥Atpsy基因的T1代生菜和野生型生菜,测定各转基因株系与野生型生菜的各项生理生化指标、目的基因的表达量和类胡萝卜素的含量。结果显示:正常情况下,转基因株系与野生型植株的各项生理指标之间没有明显的差异,随胁迫程度加深,转基因株系在发芽率、发芽指数和净光合速率的下降幅度方面小于野生型;同等胁迫强度下,转基因株系的脯氨酸、可溶性糖含量和超氧化物歧化酶活性高于野生型植株;转基因株系植物体内丙二醛的快速积累程度和叶绿素降解速率小于野生型对照组。干旱胁迫处理下,转基因株系Atpsy基因的表达量和类胡萝卜素的含量均有所提高。In order to investigate the effects of exogenous Atpsy gene on transgenic lettuce（Lactuca sativa var. longifolia）under drought stress, the wild-type and transgenic lettuce plants were determined under drought stress in terms of physiological and biochemical indices, Atpsy gene expression level and carotenoid contents. The results showed that all the plants were not different in physiological indices in a normal condition, but with the stress level increasing the transgenic plants＇ germination rate, germination index and net photosynthetic rate decreased obviously less than the wild-type plants＇. At the same stress level the transgenic plants＇ proline soluble sugar content and superoxide dismutase（SOD）activity were higher than the wild-type plants＇,and the malonaldehyde accumulation and chlorophyll degradation of transgenic plants were less than the wild-type plants＇. Under drought stress the target gene expression level and carotenoid content of transgenic plants improved.

关 键 词：生菜 干旱胁迫 Atpsy基因 类胡萝卜素 生理生化指标 

分 类 号：S636.2[农业科学—蔬菜学] Q78[农业科学—园艺学]