Synergistic actions of olomoucine and bone morphogenetic protein-4 in axonal repair after acute spinal cord contusion  

作　　者：Liang Chen Jianjun Li Liang Wu Mingliang Yang Feng Gao Li Yuan 

机构地区：[1]Capital Medical University School of Rehabilitation Medicine [2]Department of Spinal and Neural Function Reconstruction,China Rehabilitation Research Center [3]Center of Neural Injury and Repair,Beijing Institute for Brain Disorders [4]Rehabilitation Center,Beijing Xiaotangshan Rehabilitation Hospital [5]Department of General Surgery,China Rehabilitation Research Center

出　　处：《Neural Regeneration Research》2014年第20期1830-1838,共9页中国神经再生研究（英文版）

基　　金：supported by a grant from the ‘Twelve Five-year Plan’ for Science & Technology Research of China,No.2012BAI34B02

摘　　要：To determine whether olomoucine acts synergistically with bone morphogenetic protein-4 in the treatment of spinal cord injury, we established a rat model of acute spinal cord contusion by impacting the spinal cord at the T8 vertebra. We injected a suspension of astrocytes derived from glial-restricted precursor cells exposed to bone morphogenetic protein-4 （GDAsBMP） into the spinal cord around the site of the injury, and/or olomoucine intraperitoneally. Olomoucine effectively inhibited astrocyte proliferation and the formation of scar tissue at the injury site, but did not prevent proliferation of GDAsBMP or inhibit their effects in reducing the spinal cord lesion cavity. Furthermore, while GDAsBMP and olomoucine independently resulted in small improve- ments in locomotor function in injured rats, combined administration of both treatments had a significantly greater effect on the restoration of motor function. These data indicate that the combined use of olomoucine and GDAsBMP creates a better environment for nerve regeneration than the use of either treatment alone, and contributes to spinal cord repair after injury.To determine whether olomoucine acts synergistically with bone morphogenetic protein-4 in the treatment of spinal cord injury, we established a rat model of acute spinal cord contusion by impacting the spinal cord at the T8 vertebra. We injected a suspension of astrocytes derived from glial-restricted precursor cells exposed to bone morphogenetic protein-4 （GDAsBMP） into the spinal cord around the site of the injury, and/or olomoucine intraperitoneally. Olomoucine effectively inhibited astrocyte proliferation and the formation of scar tissue at the injury site, but did not prevent proliferation of GDAsBMP or inhibit their effects in reducing the spinal cord lesion cavity. Furthermore, while GDAsBMP and olomoucine independently resulted in small improve- ments in locomotor function in injured rats, combined administration of both treatments had a significantly greater effect on the restoration of motor function. These data indicate that the combined use of olomoucine and GDAsBMP creates a better environment for nerve regeneration than the use of either treatment alone, and contributes to spinal cord repair after injury.

关 键 词：nerve regeneration spinal cord injury OLOMOUCINE glial-restricted precursor-derivedastrocytes glial scar cavity axonal regeneration neural regeneration 

分 类 号：R651.2[医药卫生—外科学]