邻苯二甲酸二丁酯对雌性大鼠卵巢颗粒细胞分泌功能的影响  

Dibutyl phthalate influences the secretion of rat ovarian granulose cells

作　　者：王雪金[1] 周云清[1] 罗湘闽[1] 刘瑾[2] 

机构地区：[1]福建医科大学附属第二医院妇产科,福建省泉州市362000 [2]福建医科大学公共卫生学院预防医学系,福建省福州市350108

出　　处：《中国组织工程研究》2014年第42期6779-6783,共5页Chinese Journal of Tissue Engineering Research

基　　金：福建省泉州市科技计划项目(2011Z96);福建省卫生厅青年科研课题(2012-2-52)~~

摘　　要：背景:已有研究表明邻苯二甲酸二丁酯对雄性动物生殖系统具有一定的毒性作用。目的:进一步验证邻苯二甲酸二丁酯对大鼠卵巢颗粒细胞分泌功能的影响。方法:体外培养25日龄未成熟雌性SD大鼠卵巢颗粒细胞,分别加入邻苯二甲酸二丁酯使终浓度分别为(0,5,20,80μmol/L),培养24 h。采用放射免疫方法测定雌二醇和孕酮的水平;采用实时荧光定量PCR测定大鼠颗粒细胞中细胞色素芳香化酶(P450arom)、细胞色素P450胆固醇侧链裂解酶(P450scc)mRNA的水平。结果与结论:邻苯二甲酸二丁酯染毒后颗粒细胞分泌的雌二醇及孕酮水平下降,邻苯二甲酸二丁酯可降低颗粒细胞中P450arom和P450scc mRNA的表达,且表达量与邻苯二甲酸二丁酯剂量呈反比。结果提示邻苯二甲酸二丁酯可引起雌性大鼠生殖系统的功能紊乱,对其生殖系统具有毒性作用。BACKGROUND：Studies have shown that dibutyl phthalate has certain toxicity to the reproductive system of male animals. OBJECTIVE：To investigate the effects of dibutyl phthalate on secretion of rat ovarian granulose cells. METHODS：Ovarian granulosa cells from 25-day-old female Sprague-Dawley rats were cultured in vitro and exposed to different concentrations of dibutyl phthalate （0, 5, 20, 80μmol/L） for 24 hours. Estradiol and progesterone in ovarian granulosa cells were measured by the method of radio-immunity. Expression of the cytochrome P450aromatase and P450 side chain cleavage enzyme mRNA in granulosa cells were examined by real-time PCR. RESULTS AND CONCLUSION：The levels of estradiol and progesterone in granulosa cells decreased after exposure to dibutyl phthalate. With the increase of the dibutyl phthalate concentration, the expression of P450arom and P450scc mRNA decreased gradual y. Dibutyl phthalate could induce reproductive dysfunction and toxicity to female rats.

关 键 词：组织构建 组织工程 邻苯二甲酸二丁酯 颗粒细胞 细胞色素P450胆固醇侧链裂解酶 细胞色素芳香化酶 雌二醇 孕酮 

分 类 号：R318[医药卫生—生物医学工程]