移植肾急性抗体介导排斥反应中半乳糖凝集素7的表达  

Expression of galectin-7 in acute antibody-mediated rejection after renal transplantation

作　　者：韩永[1] 郭晖[2] 蔡明[1] 钱叶勇[1] 李州利[1] 周文强[1] 王强[1] 许晓光[1] 黄海燕[1] 肖漓[1] 王新颖[1] 石炳毅[1] 

机构地区：[1]解放军第309医院全军器官移植研究所研究室,器官移植与免疫调节北京市重点实验室,北京市100091 [2]华中科技大学同济医院器官移植研究所,湖北武汉市430030

出　　处：《中国组织工程研究》2014年第42期6884-6888,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(31371395);课题名称:microRNA-29c在慢性移植物血管病中作用机制的研究~~

摘　　要：背景:半乳糖凝集素家族蛋白在移植免疫调节中的作用已被提出,目前尚未有半乳糖凝集素7检测对于肾移植后围手术期移植肾功能不全的辅助诊断,对肾移植受者而言,监测半乳糖凝集素7可能及早发现移植后肾功能异常,为临床治疗争取时间。目的:检测半乳糖凝集素7在移植肾急性抗体介导性排斥反应中的表达。方法:选取同种异体肾移植后行移植肾穿刺活检诊断为急性抗体介导性排斥反应的患者27例,同时选取同期移植后肾病理穿刺经诊断为正常的患者10例做对照。应用免疫组织化学染色法检测半乳糖凝集素7在组织中的表达情况、应用Western Blot法检测各组血清半乳糖凝集素7蛋白表达水平差异。结果与结论:免疫组织化学正常组光镜下半乳糖凝集素7表达于近曲小管上皮细胞表面微绒毛,肾小球、远曲小管、集合管和静脉未见其表达;急性抗体介导的体液性排斥反应组光镜下肾小动脉内膜水肿,管壁纤维素样坏死,肾小球肾小管淋巴细胞和单核细胞浸润,半乳糖凝集素7仅见于近曲小管上皮细胞表面微绒毛,同时动脉平滑肌也有表达,阳性度较高。急性抗体介导的体液性排斥反应组半乳糖凝集素7阳性细胞计数显著高于正常对照组(P<0.01)。Western Blot检测结果,急性抗体介导的体液性排斥反应组血清半乳糖凝集素7的表达高于正常对照组(P<0.05)。结果说明,移植肾穿刺病理活检安全可靠,对患者及移植肾无不良影响,半乳糖凝集素7检测对于肾移植后围手术期移植肾功能不全的辅助诊断具有重要的指导意义。BACKGROUND：The role of galactose lectin family proteins in transplantation immunity has been proposed, but there is currently no galectin-7 detection for auxiliary diagnosis of renal dysfunction in the perioperative period after renal transplantation. For renal transplant recipients, monitoring of galectin-7 may contribute to early diagnosis of renal dysfunction after renal transplantation, and buy time for clinical treatment. OBJECTIVE：To detect the expression of galactose-7 in acute antibody mediated rejection after renal transplantation. METHODS：Twenty-seven patients who were diagnosed as having acute antibody mediated rejection after renal transplantation by renal biopsy were enrol ed, and another 10 patients without acute antibody mediated rejection after renal transplantation were selected as controls. Immunohistochemical staining and western blot assay were used to detect expression of galectin-7 in tissue and serum, respectively. RESULTS AND CONCLUSION：Results of immunohistochemistry staining showed that under light microscope, in the control group, galectin-7 distributed in the surface microvil i of proximal tubule epithelial cells, but not in glomeruli, distal tubule, col ecting duct and vein;in the acute rejection group, renal arteriole intima edema, tube wal fibrinoid necrosis, infiltration of renal glomerulus and tubule cells and mononuclear cells were found and galectin-7 only expressed in the surface microvil i of proximal tubule epithelial cells as wel as in the arterial smooth muscle. The number of galectin-7 positive cells in the acute rejection group was significantly higher than that in the control group （P〈0.1）. Western blot assay results showed that the protein expression of serum galectin-7 in the acute rejection group was higher than that in the control group （P〈0.05）. These findings indicate that renal puncture for renal transplantation is safe and reliable, has no adverse effect on the patients and renal transplant. Galectin-7 detection has an important guiding sign

关 键 词：组织构建 组织工程 半乳糖凝集素7 肾移植 急性抗体介导性排斥反应 免疫组织化学 免疫印记 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]