斑蝥酸钠与喜树碱联用抗肿瘤效果的实验研究  

Experimental Study on the Anti-cancer Effect of Combined Usage of Sodium Cantharidinate and Camptothecin

作　　者：黄丹仪 吴转斌 黄勤 张梦杰[1] 杨桦[1] 石嘉豪[1] 盛哲津[1] 费俭[1] 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]上海南方模式生物研究中心,上海201203

出　　处：《中国细胞生物学学报》2014年第11期1519-1525,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81261120568);上海市科委项目经费(批准号:12140902100)资助的课题~~

摘　　要：斑蝥酸钠(sodium cantharidinate,SCA)和喜树碱(camptothecin,CPT)均已在临床用于肿瘤的治疗。该文就SCA与CPT联合用药在体外和体内对肿瘤细胞的生长抑制作用进行了研究。结果表明,两种药物的联合使用可增强对肺癌细胞系A549和肝癌细胞系Hep3B的体外增殖的抑制作用,且不同浓度配比下联用组的CI值均小于1,表现出了协同效应。利用肿瘤移植后的斑马鱼胚胎作为模型,研究结果证明,和单药作用相比,SCA和CPT联合应用可以显著抑制斑马鱼胚胎中A549细胞的增殖和扩散,且在实验剂量下,两者的合用对斑马鱼的胚胎发育没有明显的影响。另外,还发现CPT能够抑制斑马鱼胚胎的鳍发育,并对其血管生成产生抑制作用。该文的研究结果对于临床上联合使用SCA和CPT治疗相关肿瘤提供了有用的实验数据。Both sodium cantharidinate（SCA） and camptothecin（CPT） have been used for clinical tumor therapy.We studied the inhibition on tumor cell growth when combined SCA with CPT both in vitro and in vivo.The results showed that the combination of SCA and CPT enhanced the inhibitory effect of proliferation of A549 lung cancer cell line and Hep3 B hepatoma cell line in vitro.And the CI values of SCA in conjunction with CPT of different concentrations were less than 1,indicating a synergistic effect.Using xenograft model,our results showed that the joint application of SCA and CPT could significantly inhibit the proliferation and diffusion of A549 cells in zebrafish embryo compared with single drug treatment.The combination treatment had no obvious impact on the normal development of zebrafish in the experimental dosage.We also observed that CPT inhibited the fin development of zebrafish embryos,as well as the inhibitory effect on the angiogenesis.The results provided useful experimental data for the combination therapy for related tumors using SCA and CPT in clinic.

关 键 词：斑蝥酸钠 喜树碱 药物联用 抗肿瘤药物 

分 类 号：R285[医药卫生—中药学]