表达鸡传染性法氏囊病毒VP2蛋白的干酪乳杆菌免疫保护效力  

Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens

作　　者：林红丽[1] 侯申达 王嵩[1] 王宇鹏[1] 栾云艳 侯喜林[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]中山大学医学院,广东广州510000

出　　处：《生物工程学报》2014年第11期1679-1690,共12页Chinese Journal of Biotechnology

基　　金：黑龙江省研究生创新科研项目(No.YJSCX2013-11BYND)资助~~

摘　　要：利用干酪乳杆菌作为传染性法氏囊病毒(IBDV)VP2抗原传递系统,探讨口服雏鸡的免疫次数、免疫剂量、免疫途径和攻毒保护效果。用pLA-VP2重组干酪乳杆菌对5日龄雏鸡进行二次和三次免疫,并设108、109、1010 CFU/mL的重组干酪乳杆菌组,间接ELISA检测血清IgG和小肠洗液sIgA,末免后7 d攻毒,计算保护效果。根据确定的2次免疫和109 CFU/mL免疫剂量免疫5日龄雏鸡,分别口服、滴鼻/点眼pLA-VP2/L.casei,口服、肌注商品活苗及口服pLA/L.casei和PBS为对照,监测IgG和sIgA抗体水平;末免后7 d检测脾淋巴细胞增殖情况并攻毒,7 d后剖检,观察法氏囊损伤程度并记录病变得分和保护率。结果表明各组的特异性sIgA、IgG抗体水平显著高于对照组(P<0.01);口服pLA-VP2/L.casei组的淋巴细胞刺激指数显著高于其他组(P<0.01),保护率高达83.3%,免疫保护效果优于滴鼻/点眼组。因此,构建的重组干酪乳杆菌的安全性优于商品活苗,可以作为IBDV候选疫苗。In order to determine immunogenicity and protective effect in chickens, we used the IBDV（Infectious bursal disease virus）-Vp2/Lactobacillus casei as antigen transfer system. First, the immunized and control chickens were challenged by IBDV/DQ at lethal dose to determine the protective ratio. Second, chickens were orallyand intranasally vaccinated twice with 10^9 CFU/m L p LA-VP2/L. casei, p LA/L. casei and PBS as negativecontrol and commercial vaccine as positive control. The bursa injury and the lesion score wererecorded post challenge. The level of specific Ig G and s Ig A in p LA-VP2/L. casei and positive control groups was significantly higher than that in negativecontrol groups. The protection efficacy in p LA-VP2/L. casei oral group was higher than that inintranasal group. The SI. of p LA-VP2/L. casei oral group was significant higher than other groups. The lesion score indicated the p LA-VP2/L. casei was safer than commercial vaccine for bursa. Collectively, the p LA-VP2/L. casei could be a vaccine candidate for IBDV.

关 键 词：传染性法氏囊病毒(IBDV) VP2蛋白 重组干酪乳杆菌 黏膜免疫 免疫次数 免疫剂量 免疫途径 保护率 

分 类 号：S852.65[农业科学—基础兽医学]