两个大豆GmSBP基因的特征、亚细胞定位及对非生物胁迫的响应  

Characterization and subcellular localization of two SBP genes and their response to abiotic stress in soybean (Glycine max(L.) Merr.)

作　　者：杨艳[1] 王爽[1] 黄丽燕[1] 马洪雨[1] 舒英杰[1] 何小玲[1] 麻浩[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《生物工程学报》2014年第11期1709-1719,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.30971840;31171572;31371711);教育部高等学校博士学科点专项科研基金(Nos.20100097110030;20120097110025);上海市科技兴农推广项目(No.Hu 2013;No.1-2)资助~~

摘　　要：我国南方春大豆种子发育过程中,常处于高温、高湿季节,加之种子本身富含蛋白(约40%)和脂肪(约20%),导致南方春大豆种子易劣变。本项目组前期差异蛋白质组学研究发现蔗糖结合蛋白在高温高湿胁迫168 h时在种子田间劣变抗性品种湘豆3号发育种子中呈下调表达。为进一步从分子水平了解Gm SBP基因表达以及响应高温高湿胁迫的特性,本研究利用RT-PCR技术从大豆扩增出两个Gm SBP基因(Gm SBP2和Gm SBPL)。两个基因编码的蛋白均为亲水性,不完整的膜蛋白。荧光定量PCR分析表明:在高温高湿条件下,种子田间劣变不抗品种宁镇1号和抗性品种湘豆3号发育种子中Gm SBP2和Gm SBPL基因表达量均受高温高湿胁迫影响,也会导致种子中蔗糖和可溶性糖含量变化。在籽粒发育过程中,Gm SBP2和Gm SBPL基因表达量在花后30 d左右达到最高,对应时期的蔗糖和可溶性糖含量也达到最大值。组织特异性显示Gm SBP和Gm SBPL基因在不同组织间存在差异表达。亚细胞定位结果表明Gm SBP2和Gm SBPL蛋白均定位在细胞膜和细胞质中。以上结果表明Gm SBP2和Gm SBPL基因可能参与了植物非生物胁迫的应答过程,这将从一个侧面丰富我们对大豆种子田间劣变性和劣变抗性的认识。High temperature and humidity stress during seed growth and development of spring soybean can result in seed deterioration in South China. We isolated two genes（Gm SBP and Gm SBPL） encoding putative SBP proteins from soybean（Glycine max（L.） Merr.） to study their biological functions and response to abiotic stress,. The two SBP proteins are hydrophilic and incomplete membrane ones. Real-time quantitative（RT-PCR） analysis reveals that the expression of the two genes in the developing seeds of the seed deterioration resistant cultivar Xiangdou No. 3 and sensitive cultivar Ningzhen No. 1 was significantly affected by high temperature and humidity treatment. Meanwhile, the levels of sucrose and soluble sugar in the developing seeds of both cultivars were also affected under high temperature and humidity stress. During seed growth and development, the expression of the two genes as well as the levels of sucrose and soluble sugar reached the highest at 30 days after flower. Gm SBP2 and Gm SBPL were found to be differentially expressed in different soybean tissues. Sub-cellular localization indicated that two genes were located in cytoplasm and cell membrane. Our results indicate that Gm SBP2 and Gm SBPL might be involved in the response to abiotic stress, which will enrich our understanding of pre-harvest seed deterioration and resistance in soybean from one side.

关 键 词：大豆 SBP 基因分离 表达分析 亚细胞定位 

分 类 号：S565.1[农业科学—作物学]