HPLC测定四草通脉胶囊中槲皮素含量的不确定度评定  

Uncertainty Analysis of Quercetin Determination in Sicao Tongmai Capsules by HPLC

作　　者：杨娟[1] 孙黎[1] 安富荣[1] 王淑萍[1] 夏玲红[1] 林厚文[1] 

机构地区：[1]上海交通大学医学院附属仁济医院药剂科,上海200001

出　　处：《中国药师》2014年第12期2143-2145,共3页China Pharmacist

摘　　要：目的:分析HPLC法测定四草通脉胶囊中槲皮素含量的不确定度。方法:通过分析测量过程,确定不确定度来源,利用HPLC法测定四草通脉胶囊中槲皮素的含量,通过统计学方法,量化不确定度分量;最终确定测量结果的扩展不确定度和置信水平。结果:槲皮素扩展不确定度为0.19μg·g-1;四草通脉中槲皮素含量测定结果为(353.65±0.19)μg·g-1。结论:测量不确定度可用于四草通脉胶囊中槲皮素的标准限量的制定;测量不确定度的评定方法的确立对于中药质量标准的研究具有重要意义。Objective： To analyze the uncertainty of quercetin concentration determination in Sicao Tongmai capsules by HPLC. Methods： The source of uncertainty was confirmed by analyzing the HPLC determination process. The uncertainty components were quantified by statistics,and the extended uncertainty and confidence level were finally obtained. Results： The extended uncertainty of the measurement results was 0. 19 μg · g^-1. Quercetin concentration in Sicao Tongmai capsules was（ 353. 65 ± 0. 19） μg·g^-1. Conclusion： The uncertainty analysis method is suitable for the standard limit formulation for Sicao Tongmai capsules,and it is important to establish uncertainty analysis methods for traditional Chinese medicines.

关 键 词：四草通脉胶囊 不确定度 高效液相色谱 槲皮素 垂盆草 

分 类 号：R917[医药卫生—药物分析学]