猪带绦虫重组双歧杆菌（pGEX-TSO45W-4B）疫苗构建及鉴定  

Construction and identification of a recombinant Bifidobacteria （pGEX-TSO45W-4B） vaccine of Taenia solium

作　　者：周必英[1] 刘美辰[1] 贺莉芳[1] 

机构地区：[1]遵义医学院寄生虫学教研室,563003

出　　处：《中华地方病学杂志》2014年第6期591-595,共5页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81160206）;贵州省教育厅资助项目（2010040）

摘　　要：目的 构建和鉴定猪带绦虫重组双歧杆菌（Bb）（pGEX-TSO45W-4B）疫苗.方法 通过全基因合成猪带绦虫TSO45W-4B基因,预期该基因中的片段长度为351 bp.将该基因定向克隆到大肠埃希菌-Bb穿梭表达载体pGEX-1λT中,构建重组质粒pGEX-TSO45W-4B,电穿孔法将该质粒导入长双歧杆菌,构建猪带绦虫重组Bb（pGEX-TSO45W-4B）疫苗,通过酶切、PCR和测序进行鉴定,证实猪带绦虫重组Bb （pGEX-TSO45W-4B）疫苗中含有片段长度为351 bp的基因.结果 全基因合成351 bp的TSO45W-4B基因片段.酶切、PCR和测序鉴定证明猪带绦虫重组Bb （pGEX-TSO45W-4B）疫苗构建成功,均获得了351 bp基因片段.结论 成功构建了猪带绦虫重组Bb （pGEX-TSO45W-4B）疫苗,为该疫苗的表达及免疫原性研究奠定了基础.Objective To construct and identify a recombinant Bifidobacteria（Bb） （pGEX-TSO45W-4B） vaccine of Taenia solium.Methods The TSO45W-4B gene of Taenia solium was synthesized.It was expected that the fragment length of the gene was 351 bp.The gene was cloned into Escherichia coli-Bifidobacteria（Bb） shutde vector pGEX-1λT to construct a recombinant plasmid pGEX-TSO45W-4B.The recombinant plasmid was electroporated into Bb to construct a recombinant Bb （pGEX-TSO45W-4B） vaccine.The vaccine was identified by restriction analysis,PCR and sequencing,which would demonstrate that the constructed recombinant Bb （pGEX-TSO45W-4B） vaccine of Taenia solium contained the gene of fragment length of 351 bp.Results The TSO45W-4B gene of 351 bp was synthesized.Restriction analysis,PCR and sequencing results showed that the recombinant Bb （pGEX-TSO45W-4B） vaccine of Taenia solium was successfully constructed and contained the gene fragment of 351 bp.Conclusion The recombinant Bb （pGEX-TSO45W-4B） vaccine of Taenia solium is successfully constructed,which lays a foundation for the study of expression and immunogenicity of the vaccine.

关 键 词：猪带绦虫 重组Bb (pGEX-TSO45W-4B)疫苗 构建 鉴定 

分 类 号：R532.33[医药卫生—内科学]