何首乌营养器官结构与二苯乙烯苷的积累动态研究  

Dynamic Relations between Structural and Stilbene Glucoside Accumulating in Vegetative Organs of Polygonum multiflorum Thunb.

作　　者：王桂芹[1] 郑玉华[1] 王璐[1] 

机构地区：[1]安徽科技学院生命科学学院,凤阳233100

出　　处：《植物研究》2014年第6期787-791,共5页Bulletin of Botanical Research

基　　金：安徽省教育厅自然科学基金资助重点项目(KJ2009A166)

摘　　要：采用显微制片技术、组织化学定位和二苯乙烯苷定量分析等方法,对何首乌的各种营养器官分别进行了二苯乙烯苷的定位、含量和相应的组织结构特征研究。结果表明:二苯乙烯苷在何首乌的各类营养器官中均有分布,依据含量排序为:根状茎>块根>叶>茎。二苯乙烯苷的含量因营养器官的生长发育时期不同而发生变化(茎除外)。二苯乙烯苷在何首乌块根中的积累变化是呈直线上升趋势(4月份除外),10月末含量最为丰富;在根状茎和叶中二苯乙烯苷的变化趋势一致,8月末积累达到最大值;二苯乙烯苷在茎中含量较少,且在任何生长期内变化不明显。何首乌的块根属于异常结构,栓内层、次生韧皮部、三生韧皮部和维管射线是其积累二苯乙烯苷的主要场所;二苯乙烯苷在根状茎中的主要积累组织有栓内层、皮层、次生韧皮和维管射线;栅栏组织是叶内二苯乙烯苷积累的主要场所。We studied the relationships between the anatomical structure and the content of stilbene glucoside in the vegetative organs of Polygonum multijlorum Thunb.by histochemical localization,light microscope and quantitative analysis.The stilbene glucoside is accumulated in every vegetative organs including root tube,rhizome,stem and leaf of P.multiflorum and the content of stilbene glucoside is arranged in the order of rhizomes root tube leave stem.These vegetative organs（except for stem） at different growth stages all have different content of stilbene glucoside.The accumulation of stilbene glucoside in the root tuber has a linear growth trend（except on April） and achieves a peak value at the end of October.The stilbene glucoside content in rhizome and leave varies in a consistent trend and both accumulates to a maximum value at the end of August.The content of stilbene glucoside in the stem is very low and has no obvious changes at all growth stages.The root tube belongs to the tertiary structure and the stilbene glucoside mainly accumulates in phelloderm secondary phloem,tertiary phloem and vascular ray in the root tube.For the rhizome,the stilbene glucoside accumulates in the phelloderm,the cortex and the secondary phloem and vascular ray,and that in leaf accumulates mainly in the palisade tissue.

关 键 词：何首乌 二苯乙烯苷含量 营养器官 解剖结构 组织化学定位 

分 类 号：Q944.5[生物学—植物学] Q946.83