不同倍性桤木属植物遗传差异的AFLP分析  

AFLP Analysis of Genetic Differences among Alnus with Different Ploidies

作　　者：饶龙兵[1] 杨汉波[1,2] 郭洪英[3] 段红平[2] 陈益泰[1] 

机构地区：[1]中国林科院亚热带林业研究所,富阳311400 [2]云南农业大学,昆明650201 [3]四川省林业科学研究院,成都610081

出　　处：《植物研究》2014年第6期803-809,共7页Bulletin of Botanical Research

基　　金：十二五国家科技支撑项目桤木育种专题:抗逆生态树种桤木新品种选育技术研究(2012BAD01B0604);中央级公益性科研院所基本科研业务费专项资金资助(RISF2013010)

摘　　要：采用AFLP技术对桤木属4种不同倍性的116份材料指纹图谱进行了分析,5对引物组合共得到301个位点,282个为多态位点,多态位点百分率为93.7%。4个种群多态位点百分率为35.2%~72.4%,平均多态位点百分率为54.6%。总的Nei＇s基因多样性为0.3043,总Shannon多态信息指数为0.4578。种群间及种群内部均具有较丰富的遗传多样性,种群间的遗传差异大于种群内部的遗传差异。NJ聚类分析与主坐标分析结果一致,所有的供试材料明显的分为3个组,地理分布距离较近的种群优先聚在一起。We studied the genetic difference between species in Alnus with different ploidies by using AFLP.Five AFLP primers produced 301 bands,of which 282 were polymorphic,accounting for 93.7%.The percentage of polymorphic bands of four population was 35.2%72.4%,respectively,with an average percentage of 54.6%.The total population of genetic diversity Nei＇s was 0.3043,and total Shannon polymorphism information was 0.4578.Among and within populations all had abundant genetic diversity,and the genetic difference interspecific was higher than that of intraspecific.By NJ cluster and PCOA,the closely related populations are geographically restricted and occur in proximity to each other.

关 键 词：桤木属 AFLP分析 遗传差异 NJ聚类分析 主坐标分析 

分 类 号：Q987[生物学—遗传学]