水提取杜仲鲜皮活性成分工艺研究  

Aqueous Extraction Technology on Main Active Component from Fresh Eucommia Bark

作　　者：李媛媛[1] 项凤影[1] 高悦[1] 祖元刚[1] 路祺[1] 张莹[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2014年第6期861-865,共5页Bulletin of Botanical Research

基　　金：国家科技支撑计划课题(2012BAD21B05);青年教师自主创新基金项目(DL12BA37)

摘　　要：以杜仲鲜皮作为原料,水为提取溶剂,采用单因素实验方法探讨料液比(1:5~1:60 g·mL^(-1))、提取时间(10~120 min)和提取温度(30~90℃)对杜仲皮总固形物得率及活性成分提取率的影响,并确定单因素实验最佳提取工艺为:料液比1:20,提取时间60 min,提取温度60℃。此条件下,总固形物得率为11.61%,其中活性成分绿原酸得率为0.073%,桃叶珊瑚苷得率为0.704%,京尼平苷得率为0.122%,松脂醇二葡萄糖苷得率为0.108%。水提取杜仲皮成分,方法简单,无环境污染和溶剂回收问题,本文为杜仲鲜皮活性成分提取和开发利用提供的实验依据。We investigated the total solid and the active component yields from fresh Eucommia bark by singlefactor experiments of aqueous extraction under the liquid ratio of 1：20 to 1：60 g · mL^-1,extraction time from 10-120 min,and extraction temperature from 30℃ to 90℃.Under the optimum extraction processes of single-factor experiment,the total solid yield was 11.61%,and the extraction yields of chlorogenic acid,aucubin,geniposide and pinoresinol diglucoside were 0.073%,0.704%,0.122%and 0.108%,respectively.Aqueous extraction has advantages of simple process without environmental pollution and solvent recovery.

关 键 词：杜仲 水浴 总固形物 活性成分 

分 类 号：S685.11[农业科学—观赏园艺]