王氏连朴饮加滑石、黄芩;茵达日-4味汤对温病湿热模型、炎性反应因子IL-1β、IL-6、TNF-α的影响  

Wangshilianpu Drink Plus Talcum,Baical Skullcap Root and Yindari-4 Decoction Treating Animal Model of Warm Disease with Damp-heat Syndrome and Its Effects on the Inflammation Factors of IL-1β,IL-6 and TNF-α

作　　者：王雪峰[1] 韩雪梅[1] 任存霞[1] 

机构地区：[1]内蒙古医科大学中医学院中医临床基础教研室,呼和浩特010059

出　　处：《世界中医药》2014年第11期1524-1527,共4页World Chinese Medicine

基　　金：内蒙古自治区自然科学基金重点项目(编号:2010Zd34)

摘　　要：目的:通过动物实验观察王氏连朴饮加滑石、黄芩;茵达日-4味汤对大鼠湿热模型、炎性反应因子血清白细胞介素1β、白细胞介素6、肿瘤坏死因子α的作用影响。方法:建立大鼠湿热炎性反应模型,设空白组、模型组、对照药组、中药组、蒙药组分别采取不同处理方法观察记录各项指标,血清细胞因子IL-1β、IL-6、TNF-α检测采用酶联免疫吸附法(ELISA)。结果:中蒙医温病经典方在治疗大鼠湿热症状上具有可靠疗效,尤其在改善大鼠进食饮水减少,大便稀软,口角眼角分泌物增多等方面有显著成效。ELISA检测显示对照药组IL-1β、IL-6、TNF-α无意义,中药组TNF-α有统计学意义(P<0.01),对IL-1β、IL-6无意义(P>0.05),蒙药组TNF-α有统计学意义(P<0.01)、IL-6有意统计学义(P<0.05),对IL-1β无意义(P>0.05)。结论:王氏连朴饮加滑石、黄芩;茵达日-4味汤在解除湿热症状上有切实可靠的作用,对腹腔炎性反应引起的发热及炎性反应,虽然细胞因子血清检测没有得出有力检验数据支持,但动物模型治疗后期各项体征监测都有好转。ELISA检测显示中药组TNF-α有统计学意义(P<0.01);蒙药组TNF-α有统计学意义(P<0.01)、IL-6有统计学意义(P<0.05)。Objective：To observe Wangshilianpu drink plus talcum,baical skullcap root and Yindari-4 decoction treating rat models with damp-heat syndrome,and its effect on inflammatory factors of Factors of IL-1β,IL-6 and TNF-α.Methods：Damp heat inflammation of rats model was set up,and there were four groups of rats including the blank group,model group,control group,the Chinese medicine group,and Mongolian medicine group.Different treatments were conducted and index,serum cytokines beta,IL-6,IL-1 TNF alpha test were observed by using enzyme-linked immunosorbent （ELISA）.Results：The classic prescriptions of Chinese and Mongolian medicine treating warm diseases had reliable efficacy in the treatment of rats with damp-heat symptoms,especially in improving the reduced intake of water,runny stool and spat canthus secretion.ELISA detection showed that there was no significant improvement in the control group in terms of IL-1β,IL-6 and TNF-αbeta;Chinese traditional medicine group showed obvious difference in terms of TNF-α（P 〈0.01）,and there was no significant improvement on IL-1 beta and IL-6 （P 〉0.05）;the Mongolian group showed significant improvement on IL-6 （P 〈0.05）and TNF-αv（P 〈0.01）,and no significant difference on IL-1βv（P 〉0.05）.Conclusion：Wanglianpu drink plus talcum,ba-ical skullcap root and Yindari-4 decoction on relieving symptoms of damp-heat syndrome is practical and reliable.As for fever and inflam-mation caused by abdominal inflammation,although cytokines serum does not draw strong inspection data support,but the animal models have better physical signs in the late phase of treatment.ELISA detection shows that Chinese traditional medicine group has obvious difference in TNF alpha （P 〈0.01）,and the Mongolian medicine group has obvious improvement in TNF alpha （P 〈0.01）and signifi-cant improvement in IL-6 （P 〈0.05）.

关 键 词：王氏连朴饮加滑石 黄芩 茵达日 -4 味汤 湿热模型 IL -1β IL -6 TNF -α IL-1β IL-6 TNF-α 

分 类 号：R446.1[医药卫生—诊断学]