当归多糖与川芎嗪配伍对大鼠脑缺血再灌注后血管生成的影响  

Effects of compatibility of angelica polysaccharide and tetramethylpyrazine on angiogenesis in cerebral ischemia-reperfusion rats

作　　者：方针[1,2] 廖维靖[1,2] 雷涛[3] 王珊珊[4] 蔺俊斌[1,2] 

机构地区：[1]武汉大学中南医院康复医学科,430071 [2]澳门镜湖医院康复科 [3]武汉大学人民医院康复医学科 [4]湖北省襄阳市中心医院康复医学科

出　　处：《中国康复医学杂志》2014年第11期1006-1009,1015,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金项目(81072917)

摘　　要：目的:研究当归多糖(分子量<5ku)与川芎嗪配伍治疗对大脑中动脉阻断(MCAO)再灌注大鼠模型神经功能恢复和血管生成的作用。方法:制作大鼠MCAO再灌注模型,治疗组分别腹腔注射当归多糖、川芎嗪、当归多糖-川芎嗪配伍制剂,并设立对照组。观察造模术后4h、24h、7d三个时间点行为学评分。激光扫描共聚焦显微镜(LSCM)观察缺血侧脑组织的血流量、微血管密度。结果:治疗组行为学评分值较对照组均有降低,其中当-川配伍组差异最具显著性(P<0.05)。LSCM见脑组织中微血管呈绿色荧光充盈,治疗组脑血流量和微血管密度计数较对照组均有增高,当-川配伍组差异最具显著性(P<0.05)。结论:当归多糖、川芎嗪、当-川配伍治疗对于大鼠MCAO再灌注模型的神经功能恢复及血管生成均有促进作用,其中当-川配伍治疗效果最佳。Objective：To study the effects on neurological function and angiogenesis in middle cerebral artery occlusion（MCAO）/reperfusion rats using the drug compatibility of angelica polysaccharide（APS） molecular weight（MW）5ku and tetramethylpyrazine（TMP）.Method：Models of MCAO/reperfusion were established with modified thread embolism method. Three treatment groups were given intraperitoneal injection of APS, TMP and drug compatibility of LMW（low molecular weight）-APS and TMP, a control group was also established. Behavior scoring was proceeded to all groups of rats. Laser scanning confocal microscopy（LSCM） was used to observe the plasma perfusion and microvessel density（MVD） in ischemic brain of rats.Result：The behavior scores of three treatment groups were lower than the control group, the treatment of compatibility of LMW-APS and TMP showed significant difference（P〈0.05）. Microvessels in brain tissue were filling with green fluorescence under LSCM. Cerebral blood volume（CBV） and microvessel density（MVD） in three treatment groups were higher than those in the control group, and the APS-TMP group showed significant difference（P〈0.05）.Conclusion： In this study all the drug therapies were valid on the improvement of behavior scores and angiogenesis of rats＇ focal ischemic brain injury. Among these therapies, the treatment of compatibility of LMWAPS and TMP provided the best effect.

关 键 词：当归多糖 川芎嗪 大脑中动脉阻断 神经功能缺损 激光扫描共聚焦显微镜 

分 类 号：R285.5[医药卫生—中药学]