SDF-1对糖尿病外周血EPCs功能的影响及其与PI3K/AKT信号通路的关系  

Effects of Stromal Cell-Derived-Factor-1 on Endothelial Progenitor Cells of Peripheral Blood and Their Relationship with PI3K/AKT Signal Transduction Pathway in Patients with Diabetes

作　　者：黎金凤[1] 林安华[1] 邓颖[1] 霍亚南[1] 刘精东[1] 吴明斌[1] 王晨秀[1] 

机构地区：[1]江西省人民医院内分泌科,南昌330006

出　　处：《天津医药》2014年第11期1069-1072,I0001,共5页Tianjin Medical Journal

基　　金：江西省科技厅科技支撑计划(20111BBG70018-1)

摘　　要：目的观察基质细胞衍生因子-1(SDF-1)对糖尿病外周血内皮祖细胞(EPCs)功能的影响,探讨SDF-1对EPCs的影响是否与PI3K/AKT信号通路有关。方法采集糖尿病患者(DM组)和健康对照者(HC组)外周血30 m L,提取并培养EPCs。(1)SDF-1干预组加入100μg/L SDF-1培养液,非干预组加入EGM-2MV培养基,采用Boyden小室和体外血管生成试剂盒观察EPCs的迁移和体外血管生成能力。(2)将培养的EPCs分为空白对照组、1μg/L SDF-1组、10μg/L SDF-1组、100μg/L SDF-1组、单纯AMD3100组及100μg/L SDF-1+AMD3100组,通过Western blot法检测各组EPCs中AKT蛋白的表达水平。结果 (1)无SDF-1干预时,DM组EPCs迁移和血管形成能力低于HC组,SDF-1干预后,2组的EPCs迁移和血管形成能力均较干预前增强,但DM组增强的幅度高于HC组。(2)同一浓度下,DM组的AKT蛋白表达水平均低于HC组(均P<0.01)。无论是DM组还是HC组,AKT蛋白的表达均随着加入SDF-1浓度的增加而呈递增趋势(P<0.05);100μg/L SDF-1+AMD3100组AKT蛋白的表达水平较100μg/L SDF-1组明显降低(P<0.05)。结论 SDF-1可增强外周血EPCs迁移和血管形成能力,对糖尿病患者效果更为明显,且SDF-1对EPCs的影响与PI3K/AKT信号通路有关。Objective To observe the effects of stromal cell-derived-factor-1（SDF-1） on the function of endothelial progenitor cells（EPCs）of peripheral blood in patients with diabetes, and to discuss the effects of PI3K/AKT signaling pathway on the role of SDF-1 in EPCs. Methods The peripheral blood samples （30 mL） were collected in 10 diabetes patients （DM group） and 10 healthy controls （HC group）. （1） The 100μg/L SDF-1 was added in intervention group. EGM-2MV was added in non-intervention group. The Boyden chamber and in vitro angiogenesis kit were used to analyze the migration and in vitro angiogenesis of EPCs. （2） Cultured EPCs were divided into blank control group, 1μg/L SDF-1 group, 10μg/L SDF-1 group, 100μg/L SDF-1 group, pure AMD3100 group and 100μg/L SDF-1＋AMD3100 group. AKT protein expression levels of endothelial progenitor cells were detected by Western blot assay in each group. Results （1） Without intervention with SDF-1, EPCs’migration and angiogenesis ability were lower in DM group than those in HC group. After intervention with SDF-1, the migration and angiogenesis ability were enhanced in two groups, but the increased level was higher in DM group than that of HC group. （2） Under the same concentration, AKT protein expression level was significantly lower in DM group than that in HC group （P〈0.01）. AKT protein expression levels were increased with the increased levels of SDF-1 in DM group and HC group （P〈0.05）. AKT protein expression was significantly lower in 100μg/L SDF-1＋AMD3100 group than that of 100μg/L SDF-1 group （P〈0.05）. Conclusion SDF-1 can increase the chemotactic migration and angiogenesis ability of EPCs in peripheral blood, especially for patients with diabetes. The effects of SDF-1 on EPCs were related to the PI3K/AKT signaling pathway.

关 键 词：基质细胞衍生因子-1 内皮祖细胞 PI3K/AKT 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]