鞘内注射阿片复合纳洛酮对切口痛大鼠痛行为学及血浆胃动素的影响  

Effects of Intrathecal Injection of Opioid Compound with Low-Dose Naloxone on Pain Behavior and Blood Motilin in a Rat Model of Incisional Pain

作　　者：赵军[1] 高宝柱[1] 郑宝森[2] 曹君利[3] 

机构地区：[1]天津市第三中心医院麻醉科,300170 [2]天津医科大学第二医院疼痛治疗中心 [3]徐州医学院江苏省麻醉与镇痛应用技术重点实验室

出　　处：《天津医药》2014年第11期1084-1087,共4页Tianjin Medical Journal

基　　金：天津市卫生局科技基金资助项目(2010KZ19)

摘　　要：目的观察鞘内注射吗啡、芬太尼复合小剂量纳洛酮对切口痛大鼠痛行为学及血浆胃动素(MTL)的影响。方法取鞘内置管成功的健康雄性SD大鼠72只,随机分为6组(n=12):生理盐水(NS)组,切口痛(P)组,吗啡(5μg/kg)+芬太尼(0.25μg/kg)组(MFP组),吗啡+芬太尼+纳洛酮1(0.2 ng/kg)、2(1 ng/kg)、3(5 ng/kg)组(MFPN1、2、3组)。后5组行足跖肌切口。各组取其中6只于鞘内置管前24 h(T0)、造模前24 h(T1)、术后1 h(T2)、3 h(T3)、6 h(T4)、24 h(T5)、48 h(T6)、72 h(T7)检测机械缩足阈值(PWMT)与热缩足反射潜伏期(PWTL),剩余6只于术后6h即刻断头处死,取血浆以ELISA法检测MTL浓度。结果 MFPN2组与NS组各时点PWMT差异无统计学意义,而与NS组相比,T2、T5时点PWTL上调(P<0.05),其余各时点差异无统计学意义。P组、MFPN3组与NS组比较,T2、T3、T4时点PWMT、PWTL显著下调(P<0.05)。而MFPN3组与P组比较,T6时点PWMT及T3、T4时点PWTL下调更为显著(P<0.05)。P、MFP、MFPN1、MFPN3组与NS组比较,术后6 h MTL表达下调(P<0.05)。而MFPN2与NS组MTL表达差异无统计学意义。结论鞘内注射1 ng/kg纳洛酮抑制了吗啡+芬太尼对切口痛大鼠血浆MTL表达的影响,并能上调大鼠热辐射痛阈值,增强了阿片类药物的镇痛效果。Objective To investigate effects of intrathecal injection of morphine and fentanyl combined with low-dose naloxone on the pain behavior and the expression of blood motilin （MTL） in the rat model of incisional pain.Methods A total of 72 healthy male Sprague-Dawley rats （weight 180-220 g）, successfully intrathecally catheterized, were randomly divided into 6 groups （n=12 ）：normal saline group （NS group）, incisional pain group （P group）, morphine （5μg/kg）＋fentanyl （0.25μg/kg） group （MFP group）, morphine＋fentanyl＋naloxone （0.2 ng/kg, 1 ng/kg, 5 ng/kg） group （MFPN1, MF？PN2 and MFPN3 groups）. All groups except NS group were made the model of incisional pain on the right plantar surface. At 24-hours before intrathecal cathetherization （T0）, 24-hours before modelling （T1）, 1-hours （T2）, 3-hours （T3） , 6-hours （T4）, 24-hours （T5） , 48-hours （T6） and 72-hours （T7） after modelling respectively, paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） were detected in right hind paw in 6 rats of each group. The other 6 rats in each group were sacrificed 6-hour after operation. The plasma expression of motilin was detected by ELISA. Results Compared with NS group, the PWMT was not significantly different in all time points in MFPN2 group. The values of PWTL were significantly longer at T2 and T5 in MFPN2 group than those of NS group （P〈0.05）. The PWMT and the PWTL were significantly decreased at T2, T3 and T4 in P and MFPN3 groups than those of NS group （P〈0.05）.Compared with P group, the PWMT at T6, and PWTL at T3 and T4 were significantly decreased in MFPN3 group （P〈0.05）. The MTL at T6 was significantly decreased in P, MFP, MFPN1 and MFPN3 groups compared with that of NS group （P〈0.05）. There was no significant difference in MTL between MFPN2 group and NS group （P〉0.05）.Conclusion In the rat model of incisional pain, intrathecal injection of naloxone at 1 ng/kg can inhibit th

关 键 词：促胃动素 吗啡 纳洛酮 芬太尼 切口痛 胃肠动力 

分 类 号：R614.4[医药卫生—麻醉学]