腺苷及其受体激动剂对不稳定型心绞痛患者IL-18的影响  

Effects of Adenosine and the Selective Adenosine Receptor Agonist on the Expression of Interleukin-18 in Patient with Unstable Angina

作　　者：李永辉[1] 邢晓春[1] 张寰[1] 

机构地区：[1]天津市第四中心医院心内科CCU,300140

出　　处：《天津医药》2014年第11期1132-1134,共3页Tianjin Medical Journal

基　　金：天津市卫生局科技基金资助项目(09KZ21)

摘　　要：目的：观察腺苷及腺苷受体激动剂对不稳定型心绞痛（UAP）患者白介素（IL）-18的影响，探讨腺苷受体的作用机制。方法选取15例UAP患者（UAP组）及15例健康志愿者（对照组），采用腺苷及腺苷受体激动剂干预全血标本，双抗体夹心酶联免疫吸附法（ELISA）检测IL-18。结果低浓度腺苷（1-100μmol/L）对UAP组IL-18表达有增强效应，高浓度（1 mmol/L）有抑制效应。腺苷A1受体激动剂与A3受体激动剂对IL-18表达有增强效应，而腺苷A2a受体激动剂对IL-18表达有抑制效应，腺苷A2b受体激动剂对IL-18表达未见明显效应。结论腺苷在低浓度时对UAP患者IL-18的表达有促进作用，可能通过腺苷A1和A3受体发挥效应；高浓度时有抑制作用，可能通过腺苷A2a受体发挥作用。Objective To observe the effects of adenosine and adenosine receptor agonist on the expression of interleukin-18 （IL-18） in patients with unstable angina （UAP）, and the mechanism of adenosine receptor agonists thereof. Methods Fifteen UAP and 15 healthy volunteers were included in this study. The effects of adenosine and the selective adenosine receptor agonists on the expression of IL-18 were measured by enzyme-linked immunosorbent assay （ELISA）. Results The lower concentration of adenosine （1-100μmol/L） increased the expression of IL-18 in UAP group;whereas the higher concentration of adenosine （1 mmol/L） inhibited the expression of IL-18. The adenosine A1 receptor agonist and A3 receptor agonist increased the expression of IL-18, while the adenosine A2a receptor agonist inhibited the expression of IL-18. There was no significant effect for A2b receptor agonist on the expression of IL-18. Conclusion The lower concentrations of adenosine can enhance the expression of IL-18 through adenosine A1 and A3 receptors in UAP. The higher concentrations of adenosine can inhibit the expression of IL-18 through adenosine A2a receptors. Adenosine can promote IL-18 expression in lower concentrations.

关 键 词：心绞痛 不稳定型 白细胞介素18 腺苷 受体 腺苷A1 受体 腺苷A2 受体 腺苷A3 

分 类 号：R541.42[医药卫生—心血管疾病]