链球菌溶血素O的体外精子毒性研究  

Toxicity of Streptolysin O(SLO) on the Mouse Sperm In Vitro

作　　者：施韬[1] 刘淼[1,2] 顾文文[1,2] 王鹏[1] 顾一骅[1] 陈国武[3] 

机构地区：[1]上海市计划生育科学研究所,上海200032 [2]复旦大学上海医学院,上海200011 [3]上海集爱遗传与不育诊疗中心,上海200011

出　　处：《生殖与避孕》2014年第11期887-891,共5页Reproduction and Contraception

基　　金：国家科技基础性工作专项重点项目;项目号:2013FY110533;上海市卫生局科研课题;项目号:20134180

摘　　要：目的：探讨链球菌溶血素O（streptolysin O,SLO）的体外精子毒性。方法：颈椎脱臼处死10~12周龄ICR雄性小鼠,取尾部附睾精子,将精子浓度调整到1×106/ml,并分成2份。一份与终浓度为0.2 U/ml SLO孵育15 min后作为实验组,一份不加SLO作为对照组。测定精子的运动参数以及获能后具有超激活运动状态的精子比例,并进行碘化丙啶（PI）染色和体外受精实验。结果：精子的运动参数实验组与对照组之间差异无统计学意义（P〉0.05）,实验组小鼠精子获能后的超激活现象不明显,精子本身无法恢复膜的阻隔功能,精子体外授精能力大幅下降,与对照组比,差异均有统计学意义（P〈0.05）。结论：SLO不影响精子的运动参数,但是会影响精子的超激活运动,进而大幅度降低精子的体外受精能力。Objective： To investigate the toxicity of streptolysin O （SLO） on the mouse sperm in vitro. Methods： Male mice aged 10-12 weeks were killed by cervical vertebra dislocation, and the cauda epididymis sperms were picked up. The concentration of the sperms was adjusted to 1× 106/ml and the sperms were separated into two parts. One part was treated with SLO for 15 min as experimental group, the other was used as control group. Two groups were treated with the same procedure except that experimental group which was treated by SLO firstly. The sperm motility parameters and the ratio of hyperactivated sperms after capacitation were determined, the sperms were then applied for PI staining and in vitro fertilization （IVF） was applied to evaluate the fertilization capability of the SLO-treated sperms. Results： No statistical difference was observed between the two groups in motility parameters. Compared with the control, SLO-treated mouse sperms showed a poor status of hyperactivation after capacitation, since the sperms were incapable to restore membrane barrier function. Meanwhile, with SLO treatment, the sperms demonstrated a low capability of fertilization in vitro, with a statistically significant difference （P〈0.05）. Conclusion： SLO has no effect on the motility of the sperm, but could adversely affect sperm hyperactivation, which could result in dramatically reduced fertility in a procedure of IVF.

关 键 词：链球菌溶血素O(SLO) 精子活力 碘化丙啶(PI)染色 体外受精(IVF) 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]