T型钙离子通道受体在不同年龄C57BL/6J小鼠内耳中的表达  

The expression levels of three T-type calcium channel receptors in inner ear of C57BL/6J mice with age

作　　者：潘晨[1] 于亚峰[1] 凌红阳[1] 吴文璎[1] 肖根生[1] 

机构地区：[1]苏州大学附属第一医院耳鼻咽喉科,江苏苏州215006

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第22期1791-1794,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：苏州市科技局项目(No:SYS201228)

摘　　要：目的：探讨3种T型钙离子通道受体（α1G、α1H、α1I）在不同年龄段C57BL/6J小鼠螺旋神经元及耳蜗中的表达变化。方法：随机选取30只健康C57BL/6J小鼠（6~8周10只,24~26周10只,42~44周10只）,首先使用ABR检测其听力,随后使用RT-PCR来分析3种T型钙离子通道受体（α1G、α1H、α1I）在耳蜗及螺旋神经元上的定量表达。结果：3种受体在6~8周小鼠的耳蜗和螺旋神经元上均有分布,但表达量不一致,在螺旋神经元上表达量最多的是α1H,在耳蜗上表达量最多的是α1I。随着年龄增加尤其是到了42~44周龄时,3种受体的表达呈现出明显的减少。结论：3种T型钙离子通道受体在C57BL/6J小鼠耳蜗与螺旋神经元上均有表达,随着年龄的增加受体的表达减少。Objective：To explore the expression levels of three T-type calcium channel receptors（α1G;α1H;α1I）in the cochlea and spiral ganglion neurons of C57BL/6Jmice with different ages.Method：Thirty cases of C57BL/6Jmice were divided into three groups（6~8W,24~26W,42~44W）according to the age.The expressions of three T-type calcium channel receptors were quantified by RT-PCR after hearing thresholds measured by ABR.Result：Three receptors were detected in the cochlea and spiral ganglion neurons of 6~8WC57BL/6Jmice.The quantitative results showed that the expression levels ofα1Handα1Iwere highest among three receptors in spiral ganglion neurons and in the cochlea respectively.The expression levels of three receptors significantly decreased with age,especially at the age of 42~44W.Conclusion：The expression of T-type calcium channel receptors reduced with age in the inner ear of C57BL/6Jmice.

关 键 词：T型钙离子通道 受体 耳蜗 螺旋神经元 小鼠 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]