Retarding and manipulating of DNA molecules translocation through nanopores  

作　　者：Yin Zhang Gensheng Wu Wei Si Jingjie Sha Lei Liu Yunfei Chen 

机构地区：[1]Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments,School of Mechanical Engineering,Southeast University

出　　处：《Chinese Science Bulletin》2014年第35期4908-4917,共10页

基　　金：supported by the National Basic Research Program of China(2011CB707601,2011CB707605);the National Natural Science Foundation of China(50925519,51375092)

摘　　要：Nanopores are emerging sensitive sensors that can detect and analyze single charged molecule.Nanopores present a promising approach for sequencing human genome below US$1,000 because of its superior performance,such as high throughput and low cost.However,a dominant bottleneck,that is,the high translocation speed of DNA molecules,has to be overcome.This property decreases accuracy of nanopore sensors to the single-base level.In this review,we mainly introduce the recent research works of retarding and manipulating of DNA motion through nanopores by actively control of three forces,which are the driving force,interaction force between nanopore and molecule,and exterior drag force.Lastly,conclusion and further outlook are presented on future directions of nanopore-based DNA sequencing technology.Nanopores are emerging sensitive sensors that can detect and analyze single charged molecule. Nanopores present a promising approach for sequencing human gen- ome below US$1,000 because of its superior performance, such as high throughput and low cost. However, a dominant bottleneck, that is, the high translocation speed of DNA molecules, has to be overcome. This property decreases accuracy of nanopore sensors to the single-base level. In this review, we mainly introduce the recent research works of retarding and manipulating of DNA motion through nanopores by actively control of three forces, which are the driving force, interaction force between nanopore and molecule, and exterior drag force. Lastly, conclusion and further outlook are presented pore-based DNA sequencing on future directions of nano- technology.

关 键 词：DNA分子 纳米孔 操纵 易位 传感器精度 DNA测序 延缓 纳米技术 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]