DNA sequencing technology based on nanopore sensors by theoretical calculations and simulations  

作　　者：Wei Si Yin Zhang Gensheng Wu Jingjie Sha Lei Liu Yunfei Chen 

机构地区：[1]Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments,Southeast University

出　　处：《Chinese Science Bulletin》2014年第35期4929-4941,共13页

基　　金：supported by the National Basic Research Program of China(2011CB707605);the National Natural Science Foundation of China(50925519,51375092)

摘　　要：DNA sequencing based on nanopore sensors is a promising tool for third-generation sequencing technology because of its special properties,such as revolutionized speed and low cost.With about two decades of nanopore technology development,the pioneering work has demonstrated the ability of nanopores to perform single-molecule detection and DNA sequencing.However,the microscopic mechanisms of DNA transport dynamics through nanopores remain largely unknown.Currently,DNA microscopic transport in a nanopore is difficult to characterize and several unexpected experimental observations are equivocal.This limitation can be resolved using theoretical calculations and simulations.These computational methods can monitor the entire dynamic process that DNA undergoes in solution at a single-atom resolution that can accurately unveil the mystery of DNA transport dynamics and predict certain unexpected phenomena.This paper mainly reports the recent applications of computational and simulation methods applied to the study of DNA transport through both biological and synthetic nanopores.We hope the theoretical calculations and simulations of DNA transport through nanopores can benefit the design of DNA sequencing devices.DNA sequencing based on nanopore sensors is a promising tool for third-generation sequencing technol- ogy because of its special properties, such as revolutionized speed and low cost. With about two decades of nanopore technology development, the pioneering work has dem- onstrated the ability of nanopores to perform single-mole- cule detection and DNA sequencing. However, the microscopic mechanisms of DNA transport dynamics through nanopores remain largely unknown. Currently, DNA microscopic transport in a nanopore is difficult to characterize and several unexpected experimental obser- vations are equivocal. This limitation can be resolved using theoretical calculations and simulations. These computa- tional methods can monitor the entire dynamic process that DNA undergoes in solution at a single-atom resolution that can accurately unveil the mystery of DNA transport dynamics and predict certain unexpected phenomena. This paper mainly reports the recent applications of computa- tional and simulation methods applied to the study of DNA transport through both biological and synthetic nanopores. We hope the theoretical calculations and simulations of DNA transport through nanopores can benefit the design of DNA sequencing devices.

关 键 词：DNA测序技术 可计算性 纳米孔 传感器 模拟 应用程序 原子分辨率 微观机制 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]