硫酸羟氯喹对MRL/lpr狼疮小鼠肾组织细胞因子信号抑制因子表达的影响  

Effect of hydroxychloroquine on the expression of suppressors of cytokine signaling in MRL/lpr mice kidney

作　　者：冯欣[1] 高薇[1] 

机构地区：[1]辽宁医学院附属第一医院风湿免疫科,锦州121000

出　　处：《中华风湿病学杂志》2014年第11期773-776,共4页Chinese Journal of Rheumatology

基　　金：辽宁医学院博士启动基金（Y20128013）

摘　　要：目的 实验主要研究硫酸羟氯喹对MRL/lpr狼疮小鼠肾组织细胞因子信号抑制因子（SOCS）表达的影响,探讨羟氯喹对LN的保护作用及可能机制.方法 实验分为3组：正常对照组6只;MRL/lpr组10只;羟氯喹治疗组10只.检测小鼠尿蛋白定量（24 h）、抗dsDNA抗体变化,蛋白印迹法检测各组小鼠肾组织中SOCS-1和SOCS-3蛋白的水平,各组间进行组间单因素方差分析,以P＜0.05为差异有统计学意义.结果 与对照组相比,MRL/lpr组小鼠尿蛋白定量（24 h）[（2 357±509） mg与（440±90）mg]、抗dsDNA抗体水平（128.7±32.3与14.9±1.4）均显著上升,SOCS-1和SOCS-3蛋白表达水平升高,羟氯喹治疗后,小鼠的尿蛋白定量（24 h）[（71±11）mg]、抗dsDNA抗体水平（111.8±330.1）均明显下降,SOCS-1和SOCS-3蛋白表达水平明显降低（P＜0.05）.结论 羟氯喹能够抑制SLE小鼠肾组织SOCS的表达,降低蛋白尿,减轻免疫紊乱,延缓LN的进展.Objective To study the effect of hydroxychloroquine （HCQ） on the suppressors of cytokine signaling （SOCS） in the kidney of MRL/lpr mice and evaluate the protection of HCQ on lupus nephritis.Methods Twenty-six rats were randomly divided into 3 groups：the control group which was consisted of 6 mice; the MRL/lpr group which was consisted of 10 mice; and the HCQ group which was consisted of 10.24-hour urinary protein level and anti dsDNA levels were tested.The expressions of SOCS-1 and SOCS-3 protein were determined by western blot.Analysis of variance was used to determine statistically significant differences between the two groups.A P value less than 0.05 was considered to be statistically significant.Results Compared with the control group,the levels of 24-hour urinary protein [（2 356.6±509.3） mg vs （440.3±90.1）mg] and anti ds-DNA （128.7±32.3 vs 14.9±1.4） were significantly increased in the MRL/lpr group,the expression levels of SOCS-1 and SOCS-3 protein were increased.After treated with HCQ,the levels of 24-hour urinary protein [（70.8±11.1） mg vs （2 356.6±509.3） mg] and anti-dsDNA （111.8±330.1 vs 128.7±32.3）were significantly decreased,the expression level of SOCS-1 and SOCS-3 protein decreased significantly （P〈 0.05）.Conclusion HCQ can inhibit SOCS expression in the kidney of lupus mice,reduce proteinuria and delay progression of immune disorders and lupus nephritis.

关 键 词：狼疮肾炎 细胞因子信号转导蛋白抑制因子 羟氯喹 

分 类 号：R593.242[医药卫生—内科学]