木犀草素对ICR脾细胞和S180肉瘤细胞双向调节作用的实验研究  

Bilateral Regulation of Luteolin on Spleen Cells and Sarcoma S180 Cells of ICR Mice:an Experimental Study

作　　者：廖月霞[1] 孔桂美[2] 吴克艳[3] 陶文华[2] 卜平[2] 

机构地区：[1]扬州大学医学院护理学系,江苏225001 [2]扬州大学医学院中西医结合系,江苏225001 [3]扬州市第一人民医院消化内科,江苏225000

出　　处：《中国中西医结合杂志》2014年第11期1374-1378,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30873343)

摘　　要：目的研究木犀草素对正常ICR小鼠脾细胞和S180肉瘤细胞活力的调节作用。方法木犀草素浓度为50、100、200、400μmol/L作用于ICR小鼠脾细胞和S180肉瘤细胞后,采用MTT法检测木犀草素对脾细胞和S180细胞增殖作用的影响;碘化丙啶单染流式检测细胞凋亡情况,流式细胞仪检测细胞内活性氧浓度,羟自由基、DPPH自由基清除实验测定木犀草素清除自由基活性。结果与溶媒对照组比较,200、400μmol/L木犀草素作用ICR小鼠脾细胞后细胞活力增加(P<0.05),100、200、400μmol/L木犀草素作用小鼠S180肉瘤细胞后细胞活力下降(P<0.01);200、400μmol/L木犀草素Sub-G1期脾细胞比例减少(P<0.05),200、400μmol/L木犀草素Sub-G1期S180细胞比例增加(P<0.05)。与溶媒对照组比较,50、100、200及400μmol/L木犀草素作用ICR小鼠脾细胞后内活性氧水平均升高(P<0.05),不同浓度木犀草素作用S180细胞后内活性氧水平均降低(P<0.05)。木犀草素有对羟自由基及DPPH自由基清除活性的作用,且成一定量效关系。结论木犀草素能促进小鼠脾细胞活力,抑制脾细胞凋亡和抑制S180细胞活力,促进S180细胞凋亡的双向调节作用,具有进一步研究开发价值。Objective To study the regulation of luteolin on spleen cells and sarcoma S180 cells in normal ICR mice. Methods Spleen cells and S180 cells were incubated with different concentrations of luteolin（ 50,100,200,and 400 μmol / L）. The effect of luteolin on spleen cells and sarcoma S180 cells was determined by MTT assay. The apoptosis was detected using propidium iodide staining flow cytometry. Intracellular reactive oxygen species（ ROS） was determined by flow cytometric analysis. Activities of free radicals scavenging were determined by hydroxyl radical and DPPH tests. Results Compared with the solvent control group,200 and 400 μmol / L luteolin increased the spleen cells viability（ P 〈0. 05）. Luteolin at 100,200,and 400 μmol / L decreased activities of S180 cells（ P 〈0. 01）.The proportion of sub-G1 phase spleen cells was reduced after treated with 200 and 400 μmol / L luteolin（ P 〈0. 05）.The proportion of sub-G1 phase S180 cells was elevated after treated with 200 and 400 μmol / L luteolin（ P 〈0. 05）.Compared with the solvent control group,levels of intracellular ROS in spleen cells of ICR mice all increased; levels of intracellular ROS in S180 cells all decreased after treated with 50,100,200,and 400 μmol / L luteolin（ P 〈0. 05）. Luteolin scavenged hydroxyl radical and DPPH in a dose dependent manner. Conclusion Luteolin had bilateral regulation on viability and apoptosis of spleen cells and S180 cells（ promoting the viability of spleen cells,inhibitingapoptosis of spleen cells,inhibiting the viability of S180 cells,and promoting apoptosis of S180 cells）,which was worth further study and exploration.

关 键 词：木犀草素 细胞活力 细胞凋亡 活性氧 

分 类 号：R285.5[医药卫生—中药学]