慢性阻塞性肺疾病合并2型糖尿病大鼠模型的建立  

Rat Model of Chronic Obstructive Pulmonary Disease Combined with Type 2 Diabetes Mellitus

作　　者：高凌云[1] 任辉[1] 杨恂[2] 刘晓俊[2] 陶绍华[2] 潘林海[2] 

机构地区：[1]四川省医学科学院.四川省人民医院东区呼吸科,成都610101 [2]成都大学附属医院呼吸科

出　　处：《华西医学》2014年第11期2012-2015,共4页West China Medical Journal

基　　金：四川省卫生厅科研课题(090109);四川省科技厅科研项目(2010JY0102);成都市卫生局重大科技攻关课题(0917)~~

摘　　要：目的探讨制作慢性阻塞性肺疾病(COPD)合并2型糖尿病大鼠模型的方法。方法将80只Sprague-Dawley(SD)雄性大鼠随机分成COPD组、糖尿病组、COPD+糖尿病组和正常组,每组20只。采用单纯熏香烟法制备COPD大鼠动物模型,利用链脲佐菌素诱导SD大鼠产生糖尿病,两种方法协同产生COPD+糖尿病的大鼠模型,正常组常规饲养。3个月后作常规病理检查,血糖测定。结果 COPD+糖尿病组大鼠肺组织病理学改变:支气管黏膜上皮脱落严重,肺间质及气道管壁有大量的炎性细胞浸润,肺泡结构破坏,部分融合成肺大疱。造模成功后COPD+糖尿病组的血糖水平为(27.1±1.1)mmol/L,与其他3组比较差异有统计学意义(P<0.05)。结论被动吸烟方式能够建立COPD大鼠模型,利用链脲佐菌素诱导SD大鼠产生糖尿病,两种方法协同产生COPD合并糖尿病的大鼠模型,为进一步医学研究提供动物模型。Objective To investigate the establishment of rat models with chronic obstructive pulmonary disease（COPD） combined with type 2 diabetes mellitus（DM）. Methods Eighty Sprague-Dawley（SD） male rats were randomly divided into four groups： COPD group（n=20）, DM group（n=20）, COPD combined with DM group（n=20） and normal group（n=20）. COPD rats were established by cigarette smoke. Type 2 diabetes rats were modeled by streptozotocin injection. COPD combined with DM rats were modeled by cigarette smoking and streptozotocin injection at the same time. Pathological examination and blood glucose were tested after three months. Results Bronchial epithelium was seriously shedding in COPD＋DM group, with alveolar structure damaged and some alveolar fused into bullae. The blood glucose level in COPD＋DM group was（27.1 ± 1.1） mmol/L,which was statistically different from other groups（P 〈 0.05）. Conclusion Rat model of COPD combined with type 2 DM could be established by cigarette smoking and streptozotocin injection, which can provide an animal model for further medical research.

关 键 词：吸烟 慢性阻塞性肺疾病 糖尿病 大鼠 

分 类 号：R563.9[医药卫生—呼吸系统] R-332[医药卫生—内科学]