肠道NK细胞在清除鼠伤寒沙门氏菌中的作用  

The critical role of intestinal NK cells in the clearance of Salmonellaty phimurium

作　　者：张鸿儒[1] 左增妍 韩秋菊[1] 张建[1] 张彩[1] 

机构地区：[1]山东大学药学院免疫药理与免疫治疗研究所,济南250012

出　　处：《现代免疫学》2014年第6期445-451,共7页Current Immunology

基　　金：国家自然科学基金(81273220;31200651);国家重点基础研究发展计划(973)(2013CB944901)

摘　　要：为了探讨肠道固有层NK细胞在抵抗肠道胞内菌感染中作用,我们建立了鼠伤寒沙门氏菌肠道感染模型,发现在用抗体清除NK1.1+NK细胞后,小鼠体重明显下降,肠系膜淋巴结和肝脾等部位的载菌量显著增多;继而应用流式细胞技术检测到感染后小肠固有层中NK细胞数量明显增加,发生明显活化,且IFN-γ的表达量增多;最后我们观察到,清除NK细胞明显削弱小鼠肠道固有层巨噬细胞的杀菌能力。结果表明,肠道NK细胞在抵抗肠道病原微生物入侵时发挥关键的作用,并且可通过促进肠道固有层巨噬细胞的活化增强巨噬细胞对病原菌的清除作用。To explore the role of intestinal NK cells in the defense against intracelllular bacteria infection,an intestinal Salmonella infection model was established with S.typhimuriumstrain.In vivo depletion of NK cells with anti-NK1.1antibody resulted in severe loss of weight and a significant increase of bacteria load in the mesentery lymphoid nodes（MLN）,liver and spleen.The number of NK cells and the frequency of activated NK cells in the small intestine augmented and the production of IFN-γincreased after S.typhimuriuminfection.Moreover,depletion of NK cells led to significant attenuation of macrophage activation and production of bactericidal substances,such as ROS and IL-1βby macrophages.These results showed that intestinal NK cells are necessary for reducing pathogen burden and contribute to protection against the Salmonella typhimurium through promoting the activation and function of macrophages.

关 键 词：NK细胞 鼠伤寒沙门氏菌 巨噬细胞 肠道黏膜免疫系统 

分 类 号：R392.5[医药卫生—免疫学]