沉默c-myc基因对Raji细胞自噬活性的影响  

Influence on autophagic activity in Raji cells by silencing c-myc gene

作　　者：张诚[1] 

机构地区：[1]南阳医学高等专科学校第一附属医院血液科,南阳473000

出　　处：《现代免疫学》2014年第6期497-500,共4页Current Immunology

摘　　要：为了研究沉默c-myc基因后Raji细胞自噬活性的变化及其机制,采用脂质体转染SiRNA法沉默c-myc基因,在透射电镜下观察细胞自噬体的形成情况,采用western blot法测定LC3-Ⅰ、LC3-Ⅱ、Beclin-1、磷酸化P70、磷酸化AKT蛋白的表达。研究发现沉默c-myc基因后,电镜下观察到Raji细胞自噬活性随时间而增强,LC3-Ⅰ、LC3-Ⅱ蛋白表达、LC3-Ⅱ/LC3-Ⅰ比值随时间而增高;Beclin-1表达增高,磷酸化P70表达降低,磷酸化AKT表达无明显变化。结果提示沉默c-myc基因使Raji细胞自噬活性明显增强,其机制是通过上调Beclin-1表达和抑制mTOR的活性来实现的。To study the variation of autophagic activity in Raji cells after silencing c-myc gene and its mechanisms.We silenced c-myc gene by siRNA liposome transfection,observed the formation of autophagic bodies by transmission electron microscope,and detected variation of protein expression of LC3-Ⅰ,LC3-Ⅱ,Beclin-1,Phospho-P70,Phospho-AKT by western blot.After silencing c-myc gene,we discovered the autophagic activity of Raji cells enhanced over time by electron microscope,the expression of LC3-Ⅰand LC3-Ⅱ,the LC3-Ⅱ/LC3-Ⅰratio increased with time,the expression of Beclin-1increased,the expression of Phospho-P70 decreased,the expression of Phospho-AKT was not significantly changed.The results showed that silencing cmyc gene can enhance the autophagic activity of Raji cells by upregulating Beclin-1expression and inhibiting the activity of mTOR.

关 键 词：C-MYC基因 沉默 RAJI细胞 自噬 

分 类 号：R392.11[医药卫生—免疫学]