多聚左旋精氨酸通过p38/MAPK信号通路诱导NCI-H292细胞分泌IL-6、IL-8细胞  

NCI-H292 cells stimulated by poly-L-arginine release cytokines IL-6and IL-8 via p38/MAPK signaling pathway

作　　者：陆兆双[1] 范晓云[1] 陈冰[1] 张玲玲[1] 

机构地区：[1]安徽医科大学第一附属医院老年呼吸内科,合肥230022

出　　处：《安徽医科大学学报》2014年第12期1693-1696,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金青年科学基金项目(编号:81100027);安徽省高校省级自然科学研究项目(编号:KJ2011A176);安徽医科大学第一附属医院博士启动基金;安徽省人社厅第九批学术和技术带头人及后备人选学术科研活动资助

摘　　要：目的研究多聚左旋精氨酸(PLA)诱导气道上皮细胞NCI-H292分泌炎症因子白介素-6(IL-6)和白介素-8(IL-8)的机制。方法将NCI-H292细胞按PLA浓度分组(0、2.5、5、10、20 mg/L),采用荧光定量PCR(qRT-PCR)方法检测细胞中IL-6和IL-8 mRNA表达水平;按PLA加药时间分组(0、15、30、45、60 min),Western blot法检测p38/MAPK磷酸化水平;按对照组、PLA组、PLA+SB203580组和SB203580组分组,采用qRT-PCR法和ELISA法检测IL-6和IL-8的mRNA和蛋白表达量。结果 PLA能诱导NCI-H292细胞IL-6、IL-8 mRNA表达上调(P<0.01),PLA也能增加NCI-H292细胞p38/MAPK磷酸化水平(P<0.01),p38/MAPK阻断剂SB203580可抑制PLA诱导的NCI-H292细胞IL-6、IL-8 mRNA表达上调(P<0.01)及p38/MAPK磷酸化水平的增加(P<0.01)。结论 p38/MAPK信号通路参与PLA诱导NCI-H292细胞炎症因子IL-6和IL-8 mRNA转录过程。Objective To study the mechanism which cytokines IL-6 and IL-8 release in NCI-H292 cells were stimulated by poly-L-arginine （PLA）.Methods NCI-H292 cells were grouped according to PLA different concentrations （0,2.5,5,10,20 mg/L）.mRNA expression levels of IL-6 and IL-8 were measured by quantitative realtime PCR （qRT-PCR）.According to different administration timing of PLA on NCI-H292 cells（0,15,30,45,60 min）,the phosphorylation of p38/MAPK in each groups were measured by western blot.We separated NCI-H292 cells into control group,PLA group,PLA ＋ SB203580 group and SB203580 group.The p38/MAPK phosporylated level was detected by Western blot.The mRNA and protein expression levels of IL-6 and IL-8 were detected by qRT-PCR and enzyme linked immunosorbent assay （ELISA）.Results PLA could induce the mRNA expressions of IL-6 and IL-8 （P ＜0.01）,and also increase the phosphorylation level of p38/MAPK in NCI-H292 cells （P ＜ 0.01）.p38/MAPK was significantly inhibited by SB203580 （P ＜0.01）.The mRNA expression levels of IL-6 and IL-8 were statistically abated by SB203580 （P ＜ 0.01）.Conclusion p38/MAPK signaling pathway is involved in the mRNA transcription processes of IL-6 and IL-8 in NCI-H292 cells stimulated by PLA.

关 键 词：哮喘 多聚左旋精氨酸 p38/MAPK 白介素-6 白介素-8 气道上皮细胞 

分 类 号：R562.25[医药卫生—呼吸系统]