提高激素性股骨头坏死兔模型动物存活率的实验研究  

The experimental study of improving the survival rate of rabbits in steriod-induced osteonecrosis of the femoral head models

作　　者：李超[1] 尚希福[1] 李旭[1] 付代杰[1] 

机构地区：[1]安徽医科大学附属省立医院骨科,合肥230001

出　　处：《安徽医科大学学报》2014年第12期1726-1729,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金(编号:KJ2013A160)

摘　　要：目的应用内毒素脂多糖(LPS)联合甲基强的松龙(MPS)建立兔股骨头坏死模型,探讨提高兔存活率的方法。方法将64只新西兰大白兔随机等分为4组:模型组(A组)、庆大霉素组(B组)、庆大霉素+泮托拉唑组(C组)和对照组(D组),每组各16只。A组、B组和C组兔建立股骨头坏死模型:单次经耳缘静脉注射LPS(10μg/kg);24 h后于臀肌注射MPS(20 mg/kg),共3次,注射间隔24 h。D组臀肌注射同等剂量的生理盐水作为对照。B组和C组于最后1次注射MPS后连续7 d以4×104U/d臀肌注射庆大霉素注射液,C组同时连续7 d以40 mg/d臀肌注射泮托拉唑注射液。结果造模期间A组、B组和C组分别有8只、5只和2只动物死亡,A、B组动物死亡原因主要为呕吐、腹泻、消化道出血等,C组动物死亡原因不明。4组动物的死亡数量差异有统计学意义(P<0.05);最后一次注射MPS 6周后,3组动物存活率分别为50.0%、68.6%、87.5%。实验期间D组未出现动物死亡。A组、B组和C组存活下来的动物经组织病理学观察均显示造模成功。结论在建立兔激素性股骨头坏死模型期间的同时,应用庆大霉素预防感染和泮托拉唑护胃,可以显著提高实验动物的存活率。Objective To establish rabbit model of steriod-induced osteonecrosis of the femoral head using lipopolysaccharide （LPS） and methylprednisolone （MPS）,and to investigate methods of improving the survival rate of rabbits.Methods A total of 64 New Zealand white rabbits were randomly and equally divided into four groups：model group （A group）,gentamycin group （B group）,gentamycin ＋ pantoprazole group （C group） and control group （D group）.Rabbits in A group,B group,C group were received one intravenous of LPS （10 μg/kg）,24 h later,three injections of MPS （20 mg/kg） were given intramuscularly at a time interval of 24 h to establish models of steriod-induced osteonecrosis of the femoral head.Rabbits in D group were only injected with the same dose of saline via the gluteus as controls.Then rabbits in B group,C group were injected with gentamycin （4 × 104 U/d） via the gluteus for 7 consecutive days after the last injection of MPS.Simultaneously,Rabbits in C group received intramuscular injection with pantoprazole （40 mg/d）.Results During the evolution of the animal model,the death number in A group,B group,C group were 8,5,2,respectively.The main causes of death in A and B group were vomiting,diarrhea,gastrointestinal bleed,etc.However,animals in C group died of unknown reasons.Significant differences in the death number were detected in four groups （P ＜ 0.05）.Six weeks after the last injection of MPS,the survival rates in A group,B group,C group were 50.0％,68.6％,87.5％,respectively.There were no dead rabbits in D group.Models were successfully established in the surviving rabbits of A group,B group,C group by the method of histopathology.Conclusion The combined use of gentamycin for infection prevention and pantoprazole for gastric mucosa protection can significantly improve the survival rate of rabbits during the establishment of rabbit model of steriod-induced osteonecrosis of the femoral head.

关 键 词：激素性股骨头坏死 动物模型 内毒素脂多糖 甲基强的松龙 庆大霉素 泮托拉唑 

分 类 号：R681.8[医药卫生—骨科学]