巨桉不同状态叶片浸提液对萝卜幼苗形态和抗性生理特性的影响  

Effects of Aqueous Extracts fromEucalyptus grandis Leaves in Different Status on Morphology and Resistance Physiology of Raphanus sativus Seedlings

作　　者：喻秀艳 胡红玲[1] 王茜[1] 陈洪[1] 胡庭兴[1] 涂利华[1] 李鹏飞[1] 

机构地区：[1]四川农业大学林学院,四川雅安625014

出　　处：《西北植物学报》2014年第11期2262-2269,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：四川省教育厅项目(13ZA0246);教育部博士点基金(20125103120018);"十二五"科技支撑计划(2011BAC09B05)

摘　　要：以珍珠岩作为基质,选择4年生巨桉(Eucalyptus grandis)嫩叶(T1)、老叶(T2)、表层凋落叶(T3)、腐解凋落叶(T4)4种状态的叶片,每种状态叶片设置3个浸提液浓度水平[分别称取风干叶片30g、15g和7.5g加入900mL蒸馏水进行浸提,以蒸馏水为对照(CK)],采用水培法研究了不同状态叶片浸提液对萝卜(Raphanus sativus)幼苗形态生长和抗性生理特性的影响。结果显示:(1)巨桉不同状态叶片浸提液显著抑制了萝卜幼苗的根长,其中嫩叶的抑制作用最强,腐解凋落叶抑制作用最弱。(2)各状态叶片浸提液处理后萝卜幼苗中过氧化氢酶(CAT)和过氧化物酶(POD)的活性均呈现升高趋势,嫩叶各浓度处理以及其他状态叶片的高浓度处理下超氧化物歧化酶(SOD)活性升高,而其余浓度处理的SOD活性降低。(3)各状态叶片浸提液处理萝卜幼苗的丙二醛(MDA)含量在低浓度处理时低于CK,其余处理下则高于CK。(4)嫩叶各浓度处理萝卜幼苗的可溶性糖(SS)含量显著高于CK,且随着老叶和表层凋落叶浸提液浓度的升高,幼苗SS含量先升后降,腐解凋落叶各浓度处理下则呈渐增的趋势;而可溶性蛋白(SP)含量则随浸提液浓度的增加而升高,且T2和T3两种状态叶片的各浓度处理与CK差异显著。研究表明,巨桉不同状态叶片浸提液对萝卜幼苗生长和抗性生理产生了强烈的抑制作用,其中以嫩叶最强,老叶和表层凋落叶次之,腐解凋落叶最弱。The allelopathic effects of Eucalyptus grandis leaves with different status on morphology and resistance physiology of Raphanus sativus seedlings（receiver plant）were studied.The seedling was breed with perlite as cultivated substrate.Different status of E.grandis leaves including tender leaves（T1）,old leaves（T2）,the leaf litter from ground surface in a 4-year-old plantation（T3）and the half-decomposed leaf litter（T4）were chosen,and their aqueous extracts were prepared by soaking different amount（30g,15 g and 7.5g,dry weight）of leaves in equal amount of water（900mL）,resulting into four concentrations for each kind of leaves.The distilled water was used as the control（CK）.The results were as follows：（1）The extracts of E.grandisleaves in different status significantly inhibited the root length of R.sativus,with theextract of tender leaves having the strongest inhibition and the half-decomposed leaf litter showing the weakest inhibition.（2）The activities of catalase（CAT）and peroxidase（POD）in R.sativus were elevated treated with all levels of all extracts.The activity of superoxide dismutase（SOD）in R.sativus was increased by exposure to the tender leaves（T1）extract regardless of the concentration,and to other extracts under the high concentration,while the activity of SOD decreased when exposed to other extracts of low concentrations.（3）The content of malondialdehyde（MDA）decreased under leaf extracts with low concentrations,while increased under those with high concentrations.（4）The soluble sugar（SS）content of the receiver plant was higher in the presence of the extract of tender leaves regardless of the concentrations,compared to CK,while with the increasing extract concentration of old leaves and half-decomposed leaves.SS content presented a decrease trend after an increase.Soluble protein（SP）content increased with the increasing concentration of all kinds of extracts,and the differences became significant under all concentrations for leave

关 键 词：巨桉叶片 萝卜 化感作用 形态 抗性生理 

分 类 号：Q945.7[生物学—植物学]