打顶前后烤烟叶片microRNAs表达差异的研究  

Research on microRNAs expression profiles in flue-cured tobacco leaves before and after topping

作　　者：杨惠娟[1] 王景[1] 王红丽[1] 危月辉[1] 史宏志[1] 

机构地区：[1]河南农业大学烟草学院国家烟草栽培生理生化研究基地烟草行业栽培重点实验室,郑州450002

出　　处：《中国烟草学报》2014年第5期110-115,共6页Acta Tabacaria Sinica

基　　金：河南省高等学校青年骨干教师资助计划项目(2011GGJS-045);河南省教育厅自然科学基础研究计划项目(2013A180474)

摘　　要：为研究打顶前后烤烟叶片中差异表达的microRNAs(miRNAs),利用Solexa测序技术对打顶前后烟草叶片的小RNA文库进行测定,分别得到4,223,982和4,993,588条unique sRNA序列读数。打顶前后叶片样品中分别有347条与337条序列与库中miRNA匹配,其中注释序列分别为83和71条。在打顶前后叶片样品中表达具有显著差异的miRNA有7条,在打顶后叶片中高表达的miRNA有miR-157和miR-156;在打顶后叶片中低表达的miRNA有miR-395,miR-159c,miR-159a,miR-394和miR-399。实验还鉴定出3条新的miRNA序列,其中Nta-miRNA*-001和Nta-miRNA*-002存在打顶前叶片样品中,Nta-miRNA*-003存在打顶后叶片中。打顶前后烤烟叶片主要miRNA的表达差异反应了打顶对烤烟叶片的生长及抗逆等应激过程的调节作用。Expression of microRNAs （miRNAs） profiles in two sRNA libraries which come from flue-cured tobacco leaves before and after topping were shown by Solexa sequencing technology. Totally 4,223,982 and 4,993,588 unique sRNA sequence reads were obtained in before and after topping leaves libraries. The matched miRNAs in before and after leave samples were 347 and 337 in which 83 and 71 were annotated sequences, respectively. Seven miRNAs were identified to be differentially expressed ones between leaves before and after topping. Compared with the expression profiles in before topping samples MiR-157 and MiR-156 were expressed at a relative high level while MiR-395,MiR-159c,MiR-159a,MiR-394 and MiR-399 were shown to be lower expressed. Three new miRNA sequence were predicted in which Nta-miRNA＊-001 and Nta-miRNA＊-002 were exist in the leave sample before topping and Nta-miRNA＊-003 was exist in the after topping sample. The differentially expressed miRNAs indicated that topping can regulate the leaf vegetative growth and resistance ability.

关 键 词：烤烟 叶片 打顶 MIRNA 

分 类 号：S572[农业科学—烟草工业]