美洲大蠊提取物CII-3体内外抗HSV-2实验研究  

Experimental Study on Anti-HSV-2 Effect of CII-3 Extracted from Periplaneta Americana in Vitro and in Vivo

作　　者：普小菲[1,2] 罗亦佳 彭丽 李娇[1,2] 曾菁[1,2] 牛春丽[1,2] 彭芳[1,2] 

机构地区：[1]大理学院药学与化学学院,云南大理671000 [2]云南省昆虫生物医药研发重点实验室,云南大理671000 [3]大理学院外国语学院,云南大理671003 [4]德宏州芒市人民医院,云南德宏678400

出　　处：《大理学院学报（综合版）》2014年第10期5-9,共5页Journal of Dali University

基　　金：云南省教育厅科研基金资助项目(ZD2011005)

摘　　要：目的:研究美洲大蠊提取物CII-3抗HSV-2作用。方法:通过MTT法和CPE法测定美洲大蠊提取物CII-3抗HSV-2活性,计算其TC50、IC50及TI。以雌性小鼠生殖器疱疹为动物模型,在小鼠阴道内注射50μL HSV-2病毒原液,1次/d,连续7 d造模。以ACV为阳性对照,采用灌胃给药治疗10 d。应用ELISA试剂盒检测小鼠血清中TNF-α、IFN-γ、IL-2的水平变化;称量小鼠免疫器官重量,计算脏器指数。结果:美洲大蠊提取物CII-3有抑制HSV-2致细胞病变的作用,其TC50值为190.55μg/m L,IC50值为28.71μg/m L,TI值为6.64μg/m L;美洲大蠊提取物CII-3能够提高小鼠血清中TNF-α、IFN-γ、IL-2的水平,同时能够使小鼠的免疫器官重量增加。结论:美洲大蠊提取物CII-3有抗HSV-2作用,其抗病毒作用可能是通过提高机体免疫功能来实现。Objective： To study the anti-HSV-2 effect of CII-3 extracted from Periplaneta Americana. Methods： Anti-HSV-2 activity of CII-3 extracted from Periplaneta Americana was observed and determined with MTT assay and cytopathic effect （CPE） assay and the value of TC50, IC50 and TI in the extract was calculated. Female mice with genital herpes were used as animal models. 50μL of HSV-2 was injected into mice vaginas once a day with 7 consecutive days. With acyclovir as the positive control, intragastric administration was adopted as treatment for 10 days. The ELISA KIT was used to test the concentration of vascular tumor necrosis factor-α （TNF-α）, Interferon-γ （IFN-γ） and Interleukin-2 （IL-2）; the immune organs were weighed to calculate the organ index. Results： CII-3 extracted from Periplaneta Americana had anti-HSV-2 activity. The TC50 value was 190.55 μg/mL, IC50 value was 28.71μg/mL and TI value was 6.64μg/mL. CII-3 had the function of increasing TNF-α, IFN-γ, IL-2 and could increase the weight of the immune organs in mice. Conclusion：CII-3 extracted from Periplaneta Americana had the effect of anti-HSV-2 by boosting the body＆#39;s immunity.

关 键 词：美洲大蠊提取物 Ⅱ型单纯疱疹病毒 抗病毒作用 

分 类 号：R978.7[医药卫生—药品]