微毛诃子树皮醇提取液半数致死量的测定及对肠肌的作用  

Fuzz Myrobalan Bark Alcohol Extract Determination of Median Lethal Dose and Its Effect on Intestinal Muscle

作　　者：李代航 朱万芸 段素英[1] 赖泳[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000

出　　处：《大理学院学报（综合版）》2014年第10期10-13,共4页Journal of Dali University

基　　金：云南省重点产业创新工程基金资助项目(2008IF012)

摘　　要：目的：研究微毛诃子树皮醇提取液对小鼠的半数致死量（LD50）以及在体肠肌的影响，并且观察其对豚鼠离体肠肌的影响。方法：按Bliss法设计实验，对小鼠灌胃给药，观察5 d内小鼠的毒性反应和死亡情况，以小鼠急性死亡率为指标，测定微毛诃子提取液对小鼠的LD50；采用炭末推进实验观察药物对在体肠肌的影响；以豚鼠离体肠肌收缩幅度、张力为指标，采用离体标本运动实验法观察微毛诃子树皮醇提液对豚鼠离体肠肌的影响及其作用机制。结果：微毛诃子树皮提取液小鼠灌胃给药的LD50为5.69 g/kg，LD50的95%的可信限为4.60~7.05 g/kg；微毛诃子树皮的醇提液对小鼠在体肠肌和豚鼠离体肠肌都具有明显的舒张作用，且组胺对微毛诃子树皮的醇提液的抑制作用具有拮抗作用。结论：微毛诃子树皮的醇提取液具有一定的毒性，同时微毛诃子树皮醇提液能在一定程度上抑制肠肌的收缩，且作用机制可能与阻断组织胺受体有关。Objective：To research the micro-ethanol extract of terminalia bark median lethal dose in mice （LD50） and the influence of intestinal muscle in vivo, and observe the effect on the intestinal muscle of Guinea pig in vitro. Methods： The experiments were designed according to Bliss. Intragastric administration and observation on the toxicity and deaths of 5 d were done in mice. With acute mortality in mice as indicators, determination of extract of terminalia LD50 for mice was done; charcoal powder and advancing experimental studied the effects of drugs on the intestinal muscle; based on isolated Guinea pig intestinal smooth contraction and tension indicator, isolated specimen movement experiment was observed by micro-terminalia bark alcohol extract on isolated intestinal muscle of Guinea pigs and its mechanism of action. Results： The LD50 of terminalia bark extract in mice was 5.69 g/kg. Its 95%confidence limit was 4.60-7.05 g/kg. Minimally the ethanol extract of terminalia bark on mice intestinal muscle in vivo and in vitro in Guinea pigs has marked the vasodilator effect of intestinal muscle and histamine inhibitory effect of ethanol extract of minimal terminalia antagonism. Conclusion：Alcohol extract of terminalia bark has some toxicity, while micro-terminalia bark alcohol extract to a certain extent inhibits intestinal muscle contractions, and mechanism of action of histamine receptor-related.

关 键 词：微毛诃子 半数致死量 肠肌 

分 类 号：R285.5[医药卫生—中药学]