过敏性鼻炎的鼻液蛋白质组学分析  

Proteomic analysis of nasal lavage fluid in patients with allergic rhinitis

作　　者：谢服役[1] 张孝认[1] 孙琦[1] 

机构地区：[1]宁波市医疗中心李惠利医院检验科,浙江宁波315041

出　　处：《国际检验医学杂志》2014年第22期3027-3029,共3页International Journal of Laboratory Medicine

摘　　要：目的应用蛋白质组学技术筛选过敏性鼻炎(AR)患者鼻腔灌洗液中的蛋白标记物。方法采集10例AR患者及20例健康对照者过敏原刺激前后的鼻腔灌洗液,用双向电泳检测其中的蛋白组成,筛选差异表达的蛋白进行质谱分析鉴定。结果共筛选出6种差异表达的蛋白,在过敏原刺激后,乳铁蛋白、载脂蛋白A1(APOA1)及α1-抗胰蛋白酶表达上调,PLUNC蛋白、结合珠蛋白、IgJ表达下调。结论筛选出的6种蛋白可能成为AR的生物标记物。Objective To screen the biomarkers in nasal lavage fluid of patients with allergic rhinitis（AR）by proteomics technology.Methods The nasal lavage fluid samples of 10 cases of AR patients and 20 cases of normal controls were collected both before and after allergen stimulation tests.Two-dimensioned gel eletrophoresis was used to detect the proteins in nasal lavage fluid samples.The proteins that showed different expression levels were identified by MALDI-TOF/TOF mass spectrometry.ResultsSix kinds of proteins differentially expressed were screened out.The expressions of lactoferrin,α1-antitrypsin,and APOA1 increased in the AR patients after being stimulated by allergens.On the contrary,the expressions of PLUNC protein,haptoglobin and IgJ decreased.Conclusion The six kinds of proteins might be the biomarkers of AR.

关 键 词：过敏性鼻炎 生物标记物 蛋白质组学 

分 类 号：R765.21[医药卫生—耳鼻咽喉科] R446.6[医药卫生—临床医学]