油松毛虫体内酶系对油松诱导抗性的响应机制  

Response Mechanisms of Pine Caterpillar Enzymatic System to Pine Induced Resistance

作　　者：张雄帅[1] 周国娜[1] 高宝嘉[1] 

机构地区：[1]河北农业大学林学院,保定071000

出　　处：《林业科学》2014年第10期181-187,共7页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30771739)

摘　　要：植物与植食性昆虫在长期的协同进化过程中形成既复杂又微妙的关系。以往关于植物对昆虫的防御反应及机制的研究较为深入,但关于昆虫对植物抗性的反防御反应近年来才受到关注,尤其是昆虫对植物诱导抗性反应的研究已有明显进展（Mainguet et al.,2008;Zeng et al.,2008）。当植物产生防御物质时昆虫能利用自身代谢系统对食物中的有害物质进行解毒和排毒,大大削弱了植物的诱导抗性在害虫防治过程中的作用强度（王琛柱等，2007），所以，在研究植物与昆虫之间的作用关系时，对昆虫本身防御机制的研究是必不可少的。To explore counter-defense response mechanism of pine caterpillar（ Dendrolinus punctatus tabulaeformis Tsai et Liu） to the induced insect-resistance of Chinese pine（ Pinus tabulaeformis）,the Chinese pine was fed by different amount pine caterpillars to obtain induced insect-resistance in Pingquan of Hebei Province,and main detoxifying enzymes,protective enzymes and digestive enzymes of pine caterpillars fed on the Chinese pine leaf were determined. The results showed that most of enzymes in the pine caterpillars changed obviously after feeding various induced resistant pine leaves.Carboxylesterase,glutathione-transferase,superoxide dismutase,peroxidase and protease activity of the lavae increased significantly with the increasing feeding stimulation. Acetylcholinesterase,lipase and amylase activity of the lavae had some change but no obviously linear variation,and acetylcholinesterase activity had no change. The results indicated that carboxyl esterase,glutathione-s-transferees,superoxide dismutase,peroxidase and protease were important enzymes with that pine caterpillar responded to the pine defense. Within a certain range,the adaptive enzymes activity in the pine caterpillar increased as the increasing of the action strength and time of the pine induced defensive products to pine caterpillar.

关 键 词：油松 油松毛虫 诱导抗性 解毒酶 保护酶 消化酶 

分 类 号：Q968[生物学—昆虫学]