人参果皂苷对糖尿病大鼠心肌病变的影响  

Myocardial protection of Ginseng fruit saponins in streptozotocin-induced diabetic rats

作　　者：赵良辰[1] 真娟 尉海涛[3] 

机构地区：[1]吉林市中心医院心内科,吉林吉林132001 [2]吉林大第一医院干部病房科 [3]吉林大学第一医院心内科

出　　处：《中国实验诊断学》2014年第11期1744-1748,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨内质网应激在糖尿病心肌病的作用及人参果皂苷的干预作用及其机制。方法将50只Wistar大鼠随机分为正常对照组(C组)10只;另40只予高脂高糖饲养4周后腹腔注射链脲佐菌素(STZ)40mg/kg×4周,成功制备糖尿病大鼠模型25只,再随机分为糖尿病模型组(D组,n=10)及人参皂苷给药组(G组,n=15,40mg/kg/d给药12周),处死大鼠,取血测定空腹血糖、总胆固醇(TC)、甘油三酯(TG)、和心肌酶水平。留取心脏组织观察心脏形态改变,Tunel法检测心肌细胞凋亡,免疫组化检测Caspase-l2蛋白表达。结果 D组空腹血糖、总胆固醇(TC)、甘油三酯(TG)、CK、CK-MB及LDH水平较空白组显著升高(均为P<0.01)。应用人参果皂苷治疗后上述指标均有下降,与D组比较有显著差异(P<0.05)。C组心肌组织未见异常改变。D组大鼠心肌细胞病变明显。G组心肌细胞异常状况得到改善。C组大鼠心肌细胞凋亡指数为(3.23±1.32)%,D组心肌细胞凋亡指数为(62.5±7.59)%,G组凋亡指数为(40.25±6.58)%,与D组比较细胞凋亡明显减少。C组大鼠心肌中Caspase-l2蛋白表达较弱,D组大鼠心肌Caspase-l2蛋白表达呈现强阳性,G组Caspase-l2蛋白表达介于二者之间。结论糖尿病时内质网应激可能参与了糖尿病心肌病的病变过程;人参果皂苷能显著保护受损的心肌,其机制可能是减轻心肌细胞内质网介导的细胞凋亡。Objective To study the effect of endoplasmic reticulum stress in diabetic cardiomyopathy and the Therapeutic effects and its mechanism of ginseng fruit saponins.Methods 50 Wistar rats were randomly divided into control group（group C,n=10）;Another 40 were fed with high fat and sugar for 4weeks,then intraperitoneally injected with strept-ozotocin（STZ）[40mg/kg×4weeks]to induce diabetes,25 diabetic rats were randomly d-ivided into diabetic model group（group D,n=10）and ginseng fruit saponin group（group G,n=15,40mg/kg/D for 12weeks）,All rats were sacrificed and blood samples were coll-ected for measuring fasting blood glucose,total cholesterol（TC）,triglyceride（TG）,and myocardial enzymes（CK,CKMB,LDH）level.Tissue samples were dyed with Hemato-xylin-eosin（HE）for histopathological examination,Myocardial cell apoptosis detected by tunel method,the expressions of caspase12 protein were detected by Immunohistoch-emical detection.Results The levels of FBG,TC,TG in group D were significantly higher than those in group C（all P〈0.01）.The above indexes were significantly reduced with the treatment of ginseng fruit saponins compared with group D（P〈0.05）.The levels of CK,CK-MB and LDH in group D is significantly higher than group C（P〈0.01）,ginseng fruit saponins can significantly decrease the above indexes,the difference was statistically significant（P〈0.01）.In group C：Myocardial cell neatly and closely arranged,rich cytopl-asm is rich and red,nucleus is located in the central of cells;No dissolution of muscle fiber,vacuoles degeneration and mononuclear cell infiltration.In group D：Disordered arrange ment of myocardial cell,the cytoplasm is distributed and relatively weak dyed.fiber breakage and irregular nucleus size were obivious.Myocardial cell abnormal conditions in group G were improved.Apoptotic cells were occasionally showed in group C,the myo cardial apoptosis index was（3.23±1.32）%,Apoptotic cells increased significantly in group D,the apoptosis index was（62

关 键 词：人参果皂苷 糖尿病心肌病 内质网应激 

分 类 号：R285.5[医药卫生—中药学]