岩大戟内酯B对小鼠内毒素血症模型的保护作用研究  

The protective effects of jolkinolide B on mouse models of endotoxemia

作　　者：王慧[1] 李晓鸥[2] 张晓伟[2] 潘晨宇[3] 刘宁[3] 

机构地区：[1]吉林省疾病预防控制中心,吉林长春130062 [2]吉林省肿瘤医院,吉林长春130012 [3]吉林大学第二医院,吉林长春130041

出　　处：《中国实验诊断学》2014年第11期1752-1754,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的研究岩大戟内酯B(jolkinolide B,JB)对内毒素血症模型小鼠的治疗作用。方法采用腹腔注射内毒素制备小鼠内毒素血症模型,观察不同脓毒JB(10、5和2.5mg/kg)对内毒素血症模型小鼠的治疗效果。用ELISA法检测LPS刺激后8h小鼠血清中炎性细胞因子TNF-α、IL-1β和IL-6的含量并检测腹腔灌洗液中白细胞数目。结果JB以浓度依赖的方式降低内毒素血症模型小鼠死亡率,并显著降低血清中炎性细胞因子TNF-α、IL-1β和IL-6含量以及腹腔灌洗液中白细胞数目。结论 JB通过抑制内毒素血症小鼠炎性细胞因子表达以及炎性细胞浸润发挥其保护作用,可以成为内毒素血症治疗的候选药物。Objective To investigate the protective effects of jolkinolide B on mouse endotoxemia.Methods The mouse model of endotoxemia were prepared by intra-peritoneal injecting lipopolysaccharide（LPS）and the protective effects of JB at the concentrations of 10,5and 2.5 mg/kg on mouse models of endotoxemia were investigated.Blood was collected to determine serum TNF-α,IL-1βand IL-6levels by ELISA at 8hafter LPS treatment.The numbers of white blood cells were determined by hemocytometer count in the peritoneum wash.Results The mortality rate of mouse model of endotoxemia was decreased by JB pretreatment in a concentration-dependent manner.The numbers of white blood cells and the serum inflammatory cytokine levels such as TNF-α,IL-1βand IL-6were decreased by JB in a dose-dependent manner.Conclusion JB exerts its protective effects on endotoxemia mouse by inhibiting the expression of inflammatory cytokines and inflammatory cells infiltration.The jolkinolide B will be the therapeutic drug candidate for endotoxemia..

关 键 词：岩大戟内酯B 内毒素血症 肿瘤坏死因子-Α 白介素-1Β 白介素-6 

分 类 号：R965[医药卫生—药理学]