RP-HPLC法同时测定利肝隆颗粒中4种成分的含量  

Simultaneous determination of four constituents content in Liganlong granules by RP-HPLC

作　　者：吴庆芝 王冬梅[1] 张维萍[1] 金秀杰 李晓倩[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第11期891-895,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立RP-HPLC法同时测定利肝隆颗粒中芒柄花素、五味子醇甲、五味子甲素、五味子乙素4种成分的含量。方法采用Diamonsil C18色谱柱（200 mm×4.6 mm,5μm）进行分离;流动相：乙腈-甲醇（体积比为85∶15）（A）-体积分数为0.1%的磷酸水溶液（B）,梯度洗脱,流速：1.0 m L·min^-1,检测波长：254 nm,柱温：30℃。结果芒柄花素、五味子醇甲、五味子甲素、五味子乙素质量浓度分别在0.060 5~0.483 8 mg·L^-1（r=0.999 5,n=5）、3.390~27.12 mg·L^-1（r=0.999 7,n=5）、0.459 8~3.678 mg·L^-1（r=0.999 7,n=5）、1.818~14.54 mg·L^-1（r=0.999 6,n=5）内与峰面积呈良好的线性关系,方法的平均回收率分别为100.2%（RSD=1.1%,n=9）、99.1%（RSD=1.4%,n=9）、100.1%（RSD=1.4%,n=9）和99.4%（RSD=1.4%,n=9）。结论该方法可用于利肝隆颗粒的质量控制。Objective To establish an RP-HPLC method for determination of formononetin,schizandrol A,γ-schizandrin and deoxyschizandrin in Liganlong granules（ traditional Chinese medicines）. Methods The HPLC separation was achieved on a Diamonsil C18（ 200 mm × 4. 6 mm,5 μm） at 30℃. The mobile phase consisted of acetonitrile-methanol（ 85： 15）- 0. 1% phosphoric acid solution with gradient elution at a flowrate of 1. 0 m L·min- 1. The UV detection wavelength was set at 254 nm. Results The linear ranges were0. 060 5- 0. 483 8 mg·L^- 1（ r = 0. 999 5,n = 5）,3. 390- 27. 12 mg·L^- 1（ r = 0. 999 7,n = 5）,0. 459 8-3. 678 mg·L^- 1（ r = 0. 999 7,n = 5）,1. 818- 14. 54 mg·L^- 1（ r = 0. 999 6,n = 5） respectively. The average recoveries（ n = 9） of formononetin,schizandrol A,γ-schizandrin and deoxyschizandrin were 100. 2%（ RSD = 1. 1%,n = 9）,99. 1%（ RSD = 1. 4%,n = 9）,100. 1%（ RSD = 1. 4%,n = 9） and 99. 4%（ RSD =1. 4%,n = 9）,respectively. Conclusions The method is simple,accurate,repeatable and can be used for quality control of Liganlong granules.

关 键 词：利肝隆颗粒 芒柄花素 五味子醇甲 五味子甲素 五味子乙素 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]